Skip to main content
SpringerLink
Log in

Expression of the carbohydrate epitope 3-fucosyl-N-acetyl-lactosamine (CD15) in the vertebrate cerebellar cortex

  • Neuroatantomy; CD15 in the Normal Brain
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Summary

The distribution of the carbohydrate epitope CD15 was investigated on paraffin sections of the brains of man and mammals (monkey, dog, rabbit, rat, mouse, dolphin), reptile, bird and fish by means of immunohistochemistry. This paper demonstrates a differential expression of the CD15 epitope in the cerebella of these various vertebrates. CD15 positivity was found on glial cells and neuronal structures. In adult brains two major distribution patterns were distinguished: one with very intense labelling of the molecular layer, for which the rat is representative, the other with very low immunoreactivity in this layer (mouse). Amongst the rodents (mouse, rat and rabbit), as well as the monkey and human, the positivity in the molecular layer could be attributed to Bergmann fibres of the Golgi epithelial cells. A typical parasagittal band pattern, present in the mouse molecular layer for CD15, which is absent in rat and rabbit molecular layer, is present during human cerebellar development. CD15 positivity on neuronal structures is found on parallel fibres in the developing human, on the lower stellate cells in the dog, and in climbing fibres of the dolphin and, presumably, the catfish too. Moreover, within the parrot cerebellum, large CD15-positive mossy fibre-like endings are found just at the infraplexiform layer.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Altman, J. (1972a) Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer.J. Comp. Neurol. 145, 353–98.

    Google Scholar 

  • Altman, J. (1972b) Postnatal development of the cerebellar cortex in the rat. III. Maturation of the components of the granular layer.J. Comp. Neurol. 145, 465–514.

    Google Scholar 

  • Barclay, A. N. (1979) Localization of the THY-1 antigen in the cerebellar cortex of rat brain by immunofluorescence during postnatal development.J. Neurochem. 32, 1249–57.

    Google Scholar 

  • Bartsch, D. &Mai, J. K. (1991) Distribution of the 3-fucosyl-N-acetyl-lactosamine (FAL) epitope in the adult mouse brain.Cell Tiss. Res. 263, 253–366.

    Google Scholar 

  • Dalchau, R., Kirley, J. &Fabre, J. W. (1980) Monoclonal antibody to human brain-granulocyte-T lymphocyte antigen probably homologous to the W 3/13 antigen of the rat.Eur. J. Immunol. 10, 745–9.

    Google Scholar 

  • Ellis, R. S. (1920) Norms for some structural changes in the human cerebellum from birth to old ageJ. Comp. Neurol. 32, 1–35.

    Google Scholar 

  • Feirabend, H. K. P. &Voogd, J. (1986) Myeloarchitecture of the cerebellum of the chicken (Fallus domesticus: an atlas of the compartmental subdivision of the cerebellar white matter).J. Comp. Neurol. 251, 44–66.

    Google Scholar 

  • Feizi, T. (1985) Demonstration by monoclonal antibodies that carbohydrate structures of glycoproteins and glycolipids are onco-developmental antigens.Nature 314, 53–7.

    Google Scholar 

  • Friede, R. L. (1973) Dating the development of human cerebellum.Acta Neuropathol. (Berl.) 23, 48–58.

    Google Scholar 

  • Garson, J. A., Beverley, P. C. L., Coakham, H. B. &Harper, E. J. (1982) Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species.Nature 298, 375–7.

    Google Scholar 

  • Gocht, A., Zeunert, G. &Löhler, J. (1991a) Differentielles Expressionmuster des Stage-specific embryonic-antigen-1 (SSEA-1) während der Kleinhirnentwicklung des Menschen.Anat. Anz. (Jena) 172, 23–90.

    Google Scholar 

  • Gocht, A., Zeunert, G., Löhler, J. &Laas, R. (1991b) Temporal and spatial expression of stage-specific embryonic antigen-1 (SSEA-1) in the human cerebellum.Clin. Neuropathol. 10, 269.

    Google Scholar 

  • Hawkes, R. &Leclerc, N. (1987) Antigenic map of the rat cerebellar cortex: the distribution of parasagittal bands as revealed by monoclonal anti-Purkinje antibody mab Q113.J. Comp. Neurol. 256, 29–41.

    Google Scholar 

  • Hochstetter, F. (1929) Die Entwicklung des Mittel- und Rautenhirns. InBeiträge zur Entwicklungsgeschichte des menschlichen. Gehirns, 2. Teil (edited byDeutick, F.), pp. 82–200. Wien, Leipzig: F. Deuticke.

    Google Scholar 

  • Hogg, N., Schlusseranko, M., Cohen, J. &Reiser, J. (1981) Monoclonal antibodies with specificity for monocytes and neurons.Cell 24, 875–84.

    Google Scholar 

  • Kemshead, J. T., Bicknell, D. &Greaves, M. F. (1981) A monoclonal antibody detecting an antigen shared by neural and granulocytic cells.Pediatr. Res. 15, 1282–6.

    Google Scholar 

  • Kerr, M. A. &McCarthy, N. C. (1985) A carbohydrate differentiation antigen of granulocytes, brain and many tumours.Biochem. Soc. Trans. 13, 424–6.

    Google Scholar 

  • Kirchmeier, P. (1978) Hirnentwicklungsstörungen bei intrauteriner Entwicklungsabweichung. Thesis, Freie Universität Berlin.

  • Kornguth, S. E., Anderson, J. W. &Scott, G. (1967) Observations on the ultrastructure of the developing cerebellum of the Macaca mulatta.J. Comp. Neurol. 130, 1–24.

    Google Scholar 

  • Kuchler, S., Joubert, R., Avellana-Adalid, V., Caron, M., Bladier, D., Vincendon, G. &Panetta, J. P. (1989) Immuno-histochemical localization of a β-galactoside-binding lectin in rat central nervous system.Dev. Neurosci. 11, 414–27.

    Google Scholar 

  • Lakke, E. A. J. F., Marani, E. &Epema, A. H. (1985) Longitudinal patterns in the development of the cerebellum.Acta Morphol. Neer-Scand. 23, 127–36.

    Google Scholar 

  • Larsell, O. (1970)The Comparative Anatomy and Histology of the Cerebellum from Monotremes through Apes (edited byJansen, J.). Minneapolis: University of Minnesota Press.

    Google Scholar 

  • Larsell, O. &Jansen, J. (1972)The Comparative Anatomy and Histology of the Cerebellum. The Human Cerebellum, Cerebellar Connections, and the Cerebellar Cortex. Minneapolis: University of Minnesota Press.

    Google Scholar 

  • Mai, J. K. &Reifenberger, G. (1988) Histribution of the carbohydrate epitope 3-fucosyl-N-acetyl-lactosamine (FAL) in the adult human brain.J. Chem. Neuroanat. 1, 255–85.

    Google Scholar 

  • Mai, J. K. &Schönlau, Ch. (1992) Age related expression patterns of the CD15 epitope in the human lateral geniculate nucleus (LGN).Histochemical J. 24, 878–89.

    Google Scholar 

  • Mai, J. K., Bartsch, D., Reifenberger, G., Wechsler, W. &Birtsch, C. H. (1991)N-Acetyl-Lactosamin Immunohistochemie markiert unterschiedliche strukturen im Kleinhim von Maus Ratte un Mensch.Anat. Anz. S. 168, 691–2.

    Google Scholar 

  • Marani, E. (1982) Topographic histochemistry of the mammalia cerebella. Thesis, Leiden.

  • Marani, E. (1986) Topographic histochemistry of the cerebellum.Progr. Histochem. Cytochem. 16, 1–169.

    Google Scholar 

  • Marani, E. (1988) The fate of cerebellar allotransplants as determined with histochemical methods.Acta Histochem. S-Band36, 315–8.

    Google Scholar 

  • Marani, E. &Feiraband, H. K. P. (1983) ACRE distribution in the developing cerebellum of the rabbit. An enzyme, histochemical and immunohistochemical study.J. Histol (Lond.)137, 429–30.

    Google Scholar 

  • Marani, E. &Tetteroo, P. A. T. (1983) A longitudinal band-pattern for the monoclonal human granlocyte antibody B4,3 in the cerebellar external granular layer of the immature rabbit.Histochem. 78, 157–61.

    Google Scholar 

  • Marani, E. &Voogd, J. (1979) The morphology of the mouse cerebellum.Acta Morphol. Neerl-Scand. 17, 33–52.

    Google Scholar 

  • Marani, E., Tetteroo, P. A. T. &Van Der Veeken, J. (1983) The ultrastructural localization of the monoclonal human granulocyte antibody B4,3 in cell suspensions of the immature rabbit cerebellum.Cell Ciol. Int. Rep. 7, 763–9.

    Google Scholar 

  • McCarthy, N., Simpson, J. R. M., Coghill, G. &Kerr, M. A. (1985) Expression in normal adult, fetal, and neoplastic tissues of a carbohydrate differentiation antigen recognised by antigranulocyte mouse monoclonal antibodies.J. Clin. Pathol. 38, 521–9.

    Google Scholar 

  • Misson, J. P., Edwards, M. A., Yamamoto, M. &Caviness, V. S. Jr (1988) Identification of radial glial cells within the developing murine central nervous system: studies based upon a new immunohistochemical marker.Dev. Brain Res. 44, 95–108.

    Google Scholar 

  • Morres, S. A., Mai, J. K. &Teckhaus, L. (1992) Expression of the CD15 epitope in the human magnocellular basal forebrain system.Histochemical J. 24, 902–9.

    Google Scholar 

  • Morris, R. J., Beech, J. N., Barber, P. C. &Raisman, G. (1985) Early stages of Purkinje cell maturation demonstrated by Thy-1 immunohistochemistry on postnatal rat cerebellum.J. Neurocytol. 14, 427–52.

    Google Scholar 

  • Muramatsu, T. (1990)Cell Surface and Differentiation. Chapman & Hall, London.

    Google Scholar 

  • Nieuwenhuys, R. (1967) Comparative anatomy of the cerebellum.Progr. Brain Res. 25, 1–93.

    Google Scholar 

  • Pelc, S., Fondu, P. &Gompel, C. (1986) Immunohistochemical distribution of glial fibrillary acidic protein, neurofilament polypeptides and neuronal specific enolase in the human cerebellum.J. Neurol. Sci. 73, 289–97.

    Google Scholar 

  • Pioro, E. P., Mai, J. K. &Cuello, A. C. (1990) Distribution of substance P- and enkephalin-immunoreactive neurons and fibers. InThe Human Nervous System (edited byPaxinos, G.) pp. 1051–94. London: Academic Press.

    Google Scholar 

  • Prasadarao, N., Tobet, S. A. &Jungalwala, F. B. (1990) Effect of different fixatives on immunocytochemical localization of HNK-1-reactive antigens in cerebellum: a method for differentiating the localization of the same carbohydrate epitope on proteins vs lipids.J. Histochem Cytochem. 38, 1193–200.

    Google Scholar 

  • Rakic, P. (1971) Neuron-glia relationship during granule cell migration in developing cerebellar cortex. A Golgi and electronmicroscopic study inMacacus rhesus.J. Comp. Neurol. 141, 283–312.

    Google Scholar 

  • Rakic, P. (1972) Extrinsic cytological determinants of basket and stellate cell dendritic pattern in the cerebellar molecular layer.J. Comp. Neurol. 146, 335–54.

    Google Scholar 

  • Rakic, P. (1973) Kinetics of proliferation and latency between final cell division and onset of differentiation of cerebellar stelate and basket neurons.J. Comp. Neurol. 147, 523–46.

    Google Scholar 

  • Rakic, P. &Sidman, R. L. (1970) Histogenesis of cortical layers in human cerebellum, particularly the lamina dissecans.J. Comp. Neurol. 139, 473–500.

    Google Scholar 

  • Reifenberger, G., Mai, J. K., Krajewski, S. &Wechsler, W. (1987) Distribution of anti-Leu-7, anti-Leu-11a and anti-Leu-M1 immunoreactivity in the brain of the adult rat.Cell Tiss. Res. 248, 305–13.

    Google Scholar 

  • Sidman, R. L. &Rakic, P. (1973) Neuronal migration, with special reference to developing human brain: a review.Brain Res. 62, 1–35.

    Google Scholar 

  • Sidman, R. L. &Rakic, P. (1982) Development of the human central nervous system. InHistology and Histopathology of the Nervous System (edited byHaymaker, W. &Adams, R. D.) pp. 3–145. Springfield, C. C. Thomas.

    Google Scholar 

  • Sinclair, C. M., Greig, D. I. &Jeffrey, P. L. (1987) The developmental appearance of Thy-1 antigen in the avian nervous system.Dev. Brain Res. 35, 43–53.

    Google Scholar 

  • Skubitz, K., Balke, J., Ball, E., Bridges, R., Buescher, E. S., Campos, L., Harvath, L., Kerr, M., Kniep, Spitalnik, P., Spitalnik, S., Skubitz, A., Thompson, J., Wick, M. &Williams, L. (1989) Report on the CD15 cluster workshop. InLeucocyte Typing IV. White Cell Differentiating Antigens (edited byKnapp, W.) pp. 800–5. (Oxford: Oxford University Press.

    Google Scholar 

  • Stults, C. L. M., Sweeley, C. C. &Macher, B. A. (1989) Glycosphingolipids: structure, biological source, and properties.Meth. Enzymol. 179, 167–214.

    Google Scholar 

  • Voogd, J. (1969) The importance of fiber connections in the comparative anatomy of the mammalian cerebellum. InNeurobiology of Cerebellar Evolution and Development (edited byLlinás, R.) pp. 493–541. Chicago: AMA-ERT.

    Google Scholar 

  • Voogd, J. (1982) The olivocerebellar projection in the cat.Exp. Brain Res. 6, 134–61.

    Google Scholar 

  • Voogd, J., Gerrits, N. M. &Marani, E. (1985) Cerebellum. InThe Rat Nervous System (edited byPaxinos, G.) Ch 11. Academic Press, Austr.

    Google Scholar 

  • Voogd, J., Feirabend, H. K. P. &Schoen, J. H. R. (1990) Cerebellum and precerebellar nuclei. InThe Human Nervous System (edited byPaxinos, G.). San Diego: Academic Press.

    Google Scholar 

  • Zecevic, N. &Rakic, P. (1976) Differentiation of Purkinje cells and their relationship to other components of developing cerebellar cortex in man.J. Comp. Neurol. 167, 27–48.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Physiology, Neuroregulation Group, University of Leiden, Wassenaarseweg 62, PO Box 9604, 2300 RC, Leiden, The Netherlands

    E. Marani

  2. Department of Neuroanatomy, University of Düsseldorf, Germany

    J. K. Mai

Authors
  1. E. Marani
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. K. Mai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Marani, E., Mai, J.K. Expression of the carbohydrate epitope 3-fucosyl-N-acetyl-lactosamine (CD15) in the vertebrate cerebellar cortex. Histochem J 24, 852–868 (1992). https://doi.org/10.1007/BF01046357

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01046357

Keywords

Search

Navigation