Summary
The broad host range vectors, pKT210, pKT212, pKT248, pKT240, pGSS33 were mobilized into Zymomonas mobilis with the help of conjugative plasmids belonging to various incompatibility groups. The vectors pKT210, pKT212, pKT248, pGSS33 were stably maintained in Zymomonas mobilis in contrast to the conjugative plasmids. Based on these results we suggest the potential usefulness of these vectors as cloning vehicles in Zymomonas mobilis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Swings, J. and De Ley, J. (1977) Bacteriol. Rev. 41, 1–46.
Rogers, P.L., Lee, K.J., Skotnicki, M.L. and Tribe, D.E. (1982) Adv. Biochem. Eng. 23, 37–84.
Baratti, J. and Bu'Lock, J.D. (1986) Biotechnol. Adv. 4, 95–115.
Rogers, P.L., Skotnicki, M.L., Lee, J.E. and Lee, J.H. (1984) CRC Crit. Rev. Biotechnol. 1, 273–288.
Ingram, L.O., Carey, V.C., Dombek, K.M., Holt, A.S., Holt, W.A., Osman, Y.A. and Walia, S.K. (1984) Biomass 6, 131–143.
Eveleigh, D.E., Stokes, H.W. and Dally, E.L. (1983) In D.L. Wise (Ed) Organic Chemical from biomass, Benjamin/Cummings Publishing Co. Inc. Mento Park, Ca.
Tonomura, K., Okamoto, T., Yasui, M; and Yanase, H. (1986) Agric. Biol. Chem. 50, 805–808.
Skotnicki, M.L., Tribe, D.E. and Rogers, P.L. (1980) Appl. Environ. Microbiol. 40, 7–12.
Dally, E.L., Stokes, H.W. and Eveleigh, D.E. (1982) Biotechnol. Lett. 4, 91–96.
Carey, V.C., Walia, S.K. and Ingram, L.O. (1983) Appl. Environ. Microbiol. 46, 1163–1168.
Browne, G.M., Skotnicki, M.L., Goodman, A.E. and Rogers, P.L. (1984) Plasmids 12, 211–214.
Goodman, A.E., Strzelecki, A.T. and Rogers, P.L. (1984) J. Biotechnol., 1, 219–227.
Buchholz, S.E and Eveleigh, D.E. (1986) Appl. Env. Microbiol., 52, 366–370.
Birnboim, H.C. and Dolly, J. (1979) Nucleic Acids Research, 7, 1513–1522.
Maniatis, T., Fritsh, E.F. and Sambrook, J. (1982) in Molecular cloning: a laboratory manual. Cold spring Harbor laboratory, Cold Spring Harbor, N.Y.
Bagdasarian, M., Lurz, R., Ruckert, B., Franklin, F.C.H., Bagdasarian, M.M., Frey, J. and Timmis, K.N. (1981) Gene 16, 237–247.
Bagdasarian, M. and Timmis, K.N. (1982) Curr. Topics Microbiol. Immunol. 96, 46–67.
Bagdasarian, M.M., Amann, E., Lurz, R., Ruckert, B. and Bagdasarian, M. (1983) Gene 26, 273–282.
Sharpe, G.S. (1984) Gene 29, 93–102.
Skotnicki, M.L., Warr, R.G., Goodman, A.E., Lee, K.J. and Rogers, P.L. (1983) Biochem. Soc. Symp. 48, 53–86.
Kushner, S.R., Bayer, H.W. and Nicosia, S. (Eds) (1978) Genetic Engineering, Elsevier North-Holland, Amsterdam, 1978, 17–23.
Datta, N., Hedges, R.W., Shaw, E.J., Sykes, and Richmond, M.H. (1971) J. Bacteriol. 108, 1244–1249.
Bennett, P.M., Grinsted, J. and Richmond, M.H. (1977) Molec. Gen. Genet. 154, 205–211.
Meynell, E. and Cooke, E. (1969) Genet. Res. 14, 309–313.
Meynell, E. and Datta, N. (1967) Nature 214, 885.
Haas, D. and Holloway, B.W. 1976 Mol. Gen. Genet. 144, 243–251.
Witchitz, J.L. and Gerbaud, G.R. (1972) Ann. Inst. Pasteur 123, 333–339.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Brestic-Goachet, N., Gunasekaran, P., Cami, B. et al. Transfer and expression of broad host range plasmids in Zymomonas mobilis. Biotechnol Lett 9, 13–18 (1987). https://doi.org/10.1007/BF01043385
Issue Date:
DOI: https://doi.org/10.1007/BF01043385