Summary
An inoculum of initially freely suspended cell aggregates ofCapsicum frutescens was immobilised in porous polyurethane foam matrices. Subsequent growth and substrate consumption of these immobilised cells in batch culture were measured and compared with those of suspension cultures. The results showed that the maximum specific growth rate of freely suspended cells was slightly higher than that of immobilised cells but the overall growth patterns and final cell yields were similar.
Similar content being viewed by others
References
Brodelius, P., Deus, B., Mosbach, K., and Zenk, M.H. (1979). FEBS Lett.103, 93.
Hall, J.L., Flowers, T.J., and Roberts, R.M. (1981). Plant Cell Structure and Metabolism, 2nd Ed., Longmann, London.
Lindsey, K., Yeoman, M.M., Black, G.M., and Mavituna, F. (1983). FEBS Lett.155, 143.
Lindsey, K. and Yeoman, M.M. (1983). in: Plant Biotechnology (Smith H. and Mantell, S. M. eds) Semin. Ser. vol. 18, Soc. Exp. Biol., London.
Schenk, R.U. and Hildebrant, A.C. (1972). Can. J. Bot.50, 199.
Schuler, M.L. (1981). Production of Secondary Metabolites from Plant Tissue Culture — Problems and Prospects, Annals. N.Y. Acad. Sci. vol. 369, pp. 65–79.
Street, H.E. (1977). Plant Tissue and Cell Culture, Botanical Monographs, vol. 11, 2nd Ed., Blackwell Scientific Publications, Oxford.
Widholm, J.M. (1972). Stain Technol.47, 189.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Mavituna, F., Park, J.M. Growth of immobilised plant cells in reticulate polyurethane foam matrices. Biotechnol Lett 7, 637–640 (1985). https://doi.org/10.1007/BF01040200
Issue Date:
DOI: https://doi.org/10.1007/BF01040200