Summary
The bacteriophages T3 and T7 are not modified and restricted byE. coli strains with different host specificity (E. coli B, K, O) in vivo. The phages code for a gene product with the ability toovercomeclassicalrestriction (ocr):ocr − mutants are subject to modification and restriction via DNA methylation vs cleavage. The T3 genome possesses recognition sites for the restriction endonuclease R.EcoB which, unless the DNA is B-specifically modified, trigger 5–7 DNA cleavages. Theocr gene function of T3 and T7 is located within the gene 0.3 region of these phages and is not identical with thesam (SAMase) function of T3. The mechanisms ofocr protection remains unclear, while it is certain that this protection by the gene 0.3 protein is exerted in the infected cell and not through “over-all” modification in the preceding growth cycle of the phage.
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Adler, S.P., Nathans, D.: Studies of SV 40 DNA. V. Conversion of circular to linear SV 40 DNA by restriction endonuclease fromE. coli B. Biochim. biophys. Acta (Amst.)299, 177–188 (1973)
Arber, W.: Host specificity of DNA produced byE. coli. V. The role of methionine in the production of host specificity. J. molec. Biol.11, 247–256 (1965)
Arber, W.: DNA modification and restriction. In: Progress in nucleic acid research and molecular biology (Cohn, W.E. ed.), Vol. 14, pp. 1–37. New York-San Francisco-London: Academic Press 1974
Arber, W., Dussoix, D.: Host specificity of DNA produced byE. coli. I. Host-controlled modification of bacteriophage lambda. J. molec. Biol.5, 18–36 (1962)
Arber, W., Yuan, R., Bickle, T.A.: Strain-specific modification and restriction of DNA in bacterial. In: Post-synthetic modification of macromolecules (Antoni, F. and Farago, A., eds.), pp. 3–22 Amsterdam-London: North Holland-American Elsevier 1975
Beier, H., Hausmann, R.: Genetic map of bacteriophage T3. J. Virol.12, 417–419 (1973)
Boyer, H.W.: Restriction and modification of DNA: enzymes and substrates. Fed. Proc.33, 1125–1127 (1974)
Cantoni, G.L.: Biological methylation: selected aspects. Ann. Rev. Biochem.44, 435–451 (1975)
Clowes, R.C., Hayes, W.: Experiments in microbial genetics. Oxford-Edinburgh: Blackwell Sci. Publ. 1968
Davis, R.W., Hyman, R.W.: A study in evolution: the DNA base sequence homology between coliphage T7 and T3. J. molec. Biol.62, 287–301 (1971)
Dussoix, D., Arber, W.: Host specificity of DNA produced byE. coli. II. Control over acceptance of DNA from infecting phage lambda. J. molec. Biol.5, 37–49 (1962)
Eskin, B., Lautenberger, J.A., Linn, S.: Host-controlled modification and restriction of bacteriophage T7 byE. coli. B J. Virol.11, 1020–1023 (1973)
Eskridge, R.W., Weinfeld, H., Paigen, K.: Susceptibility of different coliphage genomes to host-controlled variation. J. Bact.93, 835–844 (1967)
Frankel, F.R.: The absence of mature phage DNA molecules from the replicating pool of T-even-infectedE. coli. J. molec. Biol.18, 109–126 (1966)
Frankel, F.R., Batcheler, M.L., Clark, C.K.: The role of gene 49 in DNA replication and head morphogenesis in bacteriophage T4. J. molec. Biol.62, 439–463 (1971)
Gefter, M., Hausmann, R., Gold, M., Hurwitz, J.: The enzymatic methylation of RNA and DNA. X. Bacteriophage T3-induced S-adenosylmethionine cleavage. J. biol. Chem.241, 1995–2005 (1966)
Gold, M., Hausmann, R., Maitra, U., Hurwitz, J.: The enzymatic methylation of RNA and DNA. VIII. Effects of bacteriophage infection on the activity of the methylating enzymes. Proc. nat. Acad. Sci. (Wash.)52, 292–297 (1964)
Grasso, R.J., Paigen, K.: Loss of host-controlled restriction and modification of phage lambda inE. coli K12 previously infected with uv-irradiated coliphage T3. Virology38, 191–194 (1969)
Hadi, S.M., Bickle, T.A., Yuan, R.: The role of S-adenosylmethionine in the cleavage of DNA by the restriction endonuclease fromE. coli K. J. biol. Chem.250, 4159–4164 (1975)
Hausmann, R.: Synthesis of an S-adenosylmethionine-cleaving enzyme in T3-infectedE. coli and its disturbance by co-infection with enzymatically incompetent bacteriophage. J. Virol.1, 57–63 (1967)
Hausmann, R., Härle, E.: Expression of the genomes of the related phages T3 and T7. In: Procedings of the First European Biophysics Congress (Broda, E. ed.), pp. 467–498. Wien: Wiener Med. Akademie 1971
Hayward, G.S., Smith, M.G.: The chromosome of bacteriophage T5. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis. J. molec. Biol.63, 383–395 (1972)
Hercules, K., Jovanovich, S., Sauerbier, W.: Early gene expression in bacteriophage T7. I.In vivo synthesis, inactivation, and translational utilization of early mRNA's. J. Virol.17, 642–658 (1976)
Herrlich, P., Rahmsdorf, H.J., Schweiger, M.: Regulation of macromolecular synthesis by membrane changes. In: Advances in the biosciences (Raspe, G. ed.), Vol. 12, pp. 523–537 Braunschweig: Pergamon Press Vieweg 1974
Herrlich, P., Schweiger, M.: T3 and T7 bacteriophage DNA-directed enzyme synthesisin vitro. J. Virol.6, 750–753 (1970)
Hirsch-Kauffmann, M., Sauerbier, W.: Inhibition of modification and restriction for phages lambda and T1 by co-infecting T3. Molec. gen. Genet102, 89–94 (1968)
Horiuchi, K., Zinder, N.D.: Cleavage of bacteriophage f1 DNA by the restriction enzyme ofE. coli B. Proc. nat. Acad. Sci. (Wash.)69, 3220–3224 (1972)
Hyman, R.W., Brunovski, I., Summers, W.C.: A biochemical comparison of the related bacteriophages T7, ϕI, ϕII, W3I, H, and T3. Virology57, 189–206 (1974)
Krüger, D.H., Hansen, S., Schroeder, C., Presber, W.: Host-dependent modification of bacteriophage T7 and SAMase-negative T3 derivatives affecting the adsorption ability. Molec. gen. Genet.153, 107–110 (1977a)
Krüger, D.H., Presber, W., Hansen, S., Rosenthal, H.A.: Biological functions of the bacteriophage T3 SAMase gene. J. Virol.16, 453–455 (1975b)
Krüger, D.H., Presber, W., Hansen, S., Rosenthal, H.A.: Different restriction of bacteriophages T3 and T7 by P1-lysogenic cells and the role of the T3-coded SAMase. Z. allg. Mikrobiol.17, (1977b)
Krüger, D.H., Presber, W., Mann, W., Rosenthal, H.A.: Untersuchungen an T3-Phagen. III. Über einen unterschiedlichen Nährstoffmangeleffekt auf die Vermehrung der Phagen T3 und T7. Acta biol. med. germ.29, 477–482 (1972)
Krüger, D.H., Scholz, D., Rosenthal, H.A.: Untersuchungen an T3-Phagen. VII. Cross streak-Komplementierung von temperatursensitiven Mutanten des Bakteriphagen T3 under konstant nonpermissiven Bedingungen. Z. allg. Mikrobiol.15, 81–88 (1975a)
Linn, S., Lautenberger, J.A., Eskin, B., Lackey, D.: Hostcontrolled restriction and modification enzymes ofE. coli B. Fed. Proc.33, 1128–1134 (1974)
Meselson, M., Yuan, R., Heywood, J.: Restriction and modification of DNA. Ann. Rev. Biochem.41, 447–466 (1972)
Simon, M.N., Studier, F.W.: Physical mapping of the early region of bacteriophage T7 DNA. J. molec. Biol.79, 249–265 (1973)
Smith, H.O., Wilcox, K.W.: A restriction enzyme from Hemophilus influenzae. I. Purification and general properties. J. molec. Biol.51, 379–391 (1970)
Studier, F.W.: The genetics and physiology of bacteriophage T7. Virology39, 562–574 (1969)
Studier, F.W.: Gene 0.3 of bacteriophage T7 acts to overcome the DNA restriction system of the host. J. molec. Biol.94, 283–295 (1975)
Studier, F.W., Movva, N.R.: SAMase gene of bacteriophage T3 is responsible for overcroming host restriction. J. Virol.19, 136–145 (1976)
Summers, W.C., Brunovskis, I., Hyman, R.W.: The process of infection with coliphage T7. VII. Characterization and mapping of the majorin vivo transcription products of the early region. J. molec. Biol.74, 291–300 (1973)
Tai, H.T., Smith, C.A., Sharp, P.A., Vinograd, J.: Sequence heterogeneity in closed SV 40 DNA. J. Virol.9, 317–325 (1972)
Tegtmeyer, P., Macasaet, F.: SV 40 DNA synthesis: analysis by gel electrophoresis. J. Virol.10, 599–604 (1972)
Wood, W.B.: Host specificity of DNA produced byE. coli: Bacterial mutations affecting the restriction and modification of DNA. J. molec. Biol.16, 118–133 (1966)
Yuan, R., Bickle, T.A., Ebbers, W., Brack, C.: Multiple steps in DNA recognition by restriction endonuclease fromE. coli K. Nature (Lond.)256, 556–560 (1975)
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Krüger, D.H., Schroeder, C., Hansen, S. et al. Active protection by bacteriophages T3 and T7 againstE. coli B-and K-specific restriction of their DNA. Molec. Gen. Genet. 153, 99–106 (1977). https://doi.org/10.1007/BF01036001
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DOI: https://doi.org/10.1007/BF01036001