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Computer model of hemopoietic stem cell population testing a possible role of DNA synthesizing cells in proliferation control

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Zusammenfassung

Es wird ein Computer-Modell für die Stammzellpopulation vorgestellt. Die Proliferationskontrolle wird in diesem Modell nur durch eine Rückkopplung erreicht, die den Zellfluß von der G0- in die G1-Phase aufgrund der Anzahl der S-Phase-Zellen, die DNA synthetisieren, ändert. Das Verhalten des Modells wurde mit experimentellen Daten verglichen, die aus der Analyse der CFUs-Zellpopulation stammen. Es besteht eine brauchbare Übereinstimmung zwischen den errechneten und experimentellen Daten, vorausgesetzt, daß einzelne Zellen während ihres Zellzyklus die G0-Phase nicht passieren. Wenn die experimentellen CFUs-Daten von pluripotenten Stammzellen nach einer Hydroxyharnstoff-Schädigung mit den Ergebnissen des Modells verglichen werden, besteht die Notwendigkeit, die Angehrate der Milzkolonietechnik zu bestimmen.

Summary

A computer model of the stem cell population is presented. Proliferation control is achieved solely in this model by a feedback, which changes the flow of cells from the g0 state into the g1 phase according to the amount of the DNA synthesizing S phase cells. Behavior of the model was compared with experimental data available about the CFUs (colony-forming units — spleen) cell population. A reasonable agreement between simulation results and experimental data could be obtained provided that some cells do not pass through the G0 state during their cell cycle. Necessity to establish the seeding efficiency of the spleen colony technique arose when experimental CFUs data showing pluripotential stem cells response to hydroxyurea damage were compared with results obtained from the model.

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Authors and Affiliations

  1. Department of Pathological Physiology, Faculty of General Medicine, Charles University, Unemocnice 5, CSSR-128 53, Prague 2, Czechoslovakia

    E. Nečas & J. Neuwirt

  2. Hybrid Computation Laboratory, Institute for Social Medicine and Organization of Health Services, Prague, Czechoslovakia

    F. Hauser

Authors
  1. E. Nečas
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  2. F. Hauser
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  3. J. Neuwirt
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Nečas, E., Hauser, F. & Neuwirt, J. Computer model of hemopoietic stem cell population testing a possible role of DNA synthesizing cells in proliferation control. Blut 41, 335–346 (1980). https://doi.org/10.1007/BF01035489

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  • DOI: https://doi.org/10.1007/BF01035489

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