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Physiological constraints in increasing biomass concentration ofEscherichia coli B in fed-batch culture

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Summary

Fed-batch culture ofE.coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial pressure of CO2 caused the decrease of the maximum specific growth rate, whereas fermentative byproducts caused the decrease of biomass yield as well as the maximum specific growth rate.

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Authors and Affiliations

  1. Genetic Engineering Center and Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, POB 150, Chongyang, Seoul, Korea

    Jae Gu Pan & Joon Shick Rhee

  2. Division de Procédés Biotéchnologiques, Université de Téchnologie de Compièngne, 60206, Compièngne, France

    Jean M. Lebeault

Authors
  1. Jae Gu Pan
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  2. Joon Shick Rhee
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  3. Jean M. Lebeault
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Pan, J.G., Rhee, J.S. & Lebeault, J.M. Physiological constraints in increasing biomass concentration ofEscherichia coli B in fed-batch culture. Biotechnol Lett 9, 89–94 (1987). https://doi.org/10.1007/BF01032744

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