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Cryoprotective agents for frozen concentrated starters from non-bitterStreptococcus lactis strains

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Summary

Six cryoprotective agents were tested on cells harvested from 4 pH-regulated fermentations of a non-bitterS. lactis strain. Addition of 5% lactose or 5% sucrose to centrifugation pellets and a storage temperature of −40 C or −70 C resulted in the highest viability and acid-producing activity of frozen concentrated starters.

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Authors and Affiliations

  1. Departamento Bioquímica y Microbiología, Instituto Nacional Investigaciones Agrarias, Apartado 8111, 28080, Madrid, Spain

    F. J. Chavarri, M. De Paz & M. Nuñez

Authors
  1. F. J. Chavarri
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  2. M. De Paz
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  3. M. Nuñez
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Chavarri, F.J., De Paz, M. & Nuñez, M. Cryoprotective agents for frozen concentrated starters from non-bitterStreptococcus lactis strains. Biotechnol Lett 10, 11–16 (1988). https://doi.org/10.1007/BF01030016

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  • DOI: https://doi.org/10.1007/BF01030016

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