Summary
Oleic acid esterification by ethanol has been performed by an immobilized lipase fromMucor miehei in supercritical carbon dioxide and in n-hexane as solvents. In both media, determination of apparent kinetic constants has been achieved and influence of water content has been shown to be different due to various rates of water solubilities. Stability of the lipase has been proved to be correct and similar in both solvents. Inhibition by ethanol excess has been found but is greater in n-hexane. That can explain the higher initial velocities obtained in supercritical carbon dioxide for the highest ethanol concentrations.
Similar content being viewed by others
References
Chi, Y.M. and Nakamura, K. (1988).Agric. Eng. Chem. 52 (6) 1541–15.
Hammond, D.A., Karel, M. and Krukonis, V.J. (1985).Appl. Biochem. Biotechnol., 11, 393–400.
Nakamura, K., Chi, Y.M. and Yamada, Y. (1986).Chem. Eng. Commun., 45, 207–21.
Pasta, P., Mazzola, G., Carrea, G. and Riva, S. (1989).Biotechol. lett. 2 (9), 643–648.
Randolph, T.W., Clark, D.S., Blanch, H.W. and Prausnitz, J.N. (1988).Science 387–390.
Taniguchi, M., Kamihira, M. and Kobayashi, T. (1987).Agric. Biol. Chem., 51 (2) 593–594.
van Eijs,A.M.M., de Jong, J.P.L., Doddema,H.J. and Lindeboom,D.R. (1988). in “Proceedings of the International Symposium on Supercritical Fluids” 933–942. Nice.
Zaks, A., and Klibanov, A.M. (1988).J. Biol. Chem., 263 (17), 8017–8020.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Marty, A., Chulalaksananukul, W., Condoret, J.S. et al. Comparison of lipase-catalysed esterification in supercritical carbon dioxide and in n-hexane. Biotechnol Lett 12, 11–16 (1990). https://doi.org/10.1007/BF01028485
Issue Date:
DOI: https://doi.org/10.1007/BF01028485