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Rapid detection of microbial hydantoinase on solid medium

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Abstract

Hydantoin hydrolysing activity of microorganisms grown on solid complex media could be detected by an overlay assay with dihydrouracil as substrate. Positive strains developed a yellow colour around their colonies within 5 to 10 seconds after challenge with 10 uL drops of an acidic para-dimethylaminobenzaldehyde solution.

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References

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Author notes

  1. André Morin

    Present address: BioEurope, 4 impasse Didier Daurat, Z. I. Montaudran, 31400, Toulouse, France

Authors and Affiliations

  1. Gesellschaft für Biotechnologische Forschung mbH, Mascheroder Weg 1, D-3300, Braunschweig-Stöckheim, Germany

    André Morin, Werner Hummel & Maria-Regina Kula

  2. Institut für Enzymtechnologie der Universität Düsseldorf in der KFA Jülich, Postfach 20 50, D-5170, Jülich 1, Germany

    Werner Hummel

Authors
  1. André Morin
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  2. Werner Hummel
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  3. Maria-Regina Kula
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Morin, A., Hummel, W. & Kula, MR. Rapid detection of microbial hydantoinase on solid medium. Biotechnol Lett 8, 571–576 (1986). https://doi.org/10.1007/BF01028085

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  • DOI: https://doi.org/10.1007/BF01028085

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