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The influence of cyclic nucleotides on hybridoma growth and monoclonal antibody production

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Summary

The influence of cyclic AMP and cyclic GMP, known regulatory mediators of cellular response, on hybridoma growth and monoclonal antibody production is studied. The cGMP-treated cells exhibited 41% higher specific antibody secretion rate, resulting in 52% higher antibody yields. Addition of 1 mM cAMP inhibited cellular growth but enhanced the specific production rate by 37%.

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Authors and Affiliations

  1. Chemical Engineering Dept., North Carolina State University, 27695-7905, Raleigh, N.C.

    Mina Dalili & David F. Ollis

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  1. Mina Dalili
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  2. David F. Ollis
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Dalili, M., Ollis, D.F. The influence of cyclic nucleotides on hybridoma growth and monoclonal antibody production. Biotechnol Lett 10, 781–786 (1988). https://doi.org/10.1007/BF01027573

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