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Microbial cell responses to a non-ionic surfactant. II. Effects as assessed by fluorescein diacetate uptake

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Summary

The effects of a non-ionic surfactant, Pluronic F-68, on uptake of fluorescein diacetate (FDA) into yeast cells as measured by increase in fluorescence atca. 500 nm have been studied. The rate of FDA uptake was increased almost 2.5 fold by incubating cells with up to 5% commercial grade pluronic but this depended on source and degree of purity. Dye uptake was reduced by pre-purification of pluronic but this again depended on source of material. None of the pluronic preparations had any significant effects on the rate of enzymemediated FDA hydrolysis by cell-free extracts.

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Authors and Affiliations

  1. Department of Zoology, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    Alastair T. King & Kenneth C. Lowe

  2. Department of Botany, University of Nottingham, University Park, NG7 2RD, Nottingham, UK

    Bernard J. Mulligan

Authors
  1. Alastair T. King
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  2. Kenneth C. Lowe
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  3. Bernard J. Mulligan
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King, A.T., Lowe, K.C. & Mulligan, B.J. Microbial cell responses to a non-ionic surfactant. II. Effects as assessed by fluorescein diacetate uptake. Biotechnol Lett 10, 873–878 (1988). https://doi.org/10.1007/BF01026998

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  • DOI: https://doi.org/10.1007/BF01026998

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