Summary
Paecilomyces varioti produced an extracellular xylanase and B-xylosidase when cultured in a medium containing xylan and corn steep liquor. Xylose (2%, w/v) totally inhibited production of both enzymes. The enzymes were purified and both had a pH optimum of 4.0. The xylanase had a molecular weight of 20,000, an isoelectric point of 5.2 and was inactive on all substrates tested except xylan. The β-xylosidase, a glycoprotein, had a molecular weight of 67,000, an isoelectric point of 4.0 and had highest activity on p-nitrophenyl-β-D-xyloside. The xylanase had a Km of 49.5 mg/ml for xylan and the β-xylosidase had a Km of 5.4 mM for p-nitrophenyl-β-D-xyloside.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Andrews, P. (1964).Biochem. J., 91, 222–233.
Bernfeld, P. (1955).Methods in Enzymology 1, 149–158.
Deschamps, A.M., De Taxis du Poet, P., Bernier, R. and Desrochers, M. (1984).Third European Congress on Biotechnology, p. 37, Veilage Chemie, Munchen.
Enari, T.M. (1983). Microbial cellulases. In:Microbial enzymes and biotechnology, W.M. Fogarty, ed. p. 183, London: Applied Science Publishers.
Frederick, M.M., Kiang, C., Frederick, J.R. and Reilly, P.J. (1985).Biotech. Bioeng. 27, 525–532.
Hansen, S.A. (1975).J. Chromatogr. 107, 324–326.
Lowry, D.H. Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951).J. Biol. Chem. 193, 265–273.
Mandels, M. (1982). Cellulases. In:Annual Reports on Fermentation Processes, G.T. Tsao, ed. vol. 5, p. 35, London: Academic Press.
McGuckin, W.F. and McKenzie, B.F. (1958).Clin. Chem. 4, 476–483.
Oguntimein, G., and Reilly, P. (1980).Biotech. Bioeng. 22, 1143–1154.
Pollock, M.R. (1961).J. Gen. Microbiol. 26, 229–253.
Rapp, P. and Wagner, F. (1986).Appl. Microbiol. Biotechnol. 51, 746–752.
Reilly, P. (1981). Xylanases: structure and function. In:Trends in the biology of fermentation for fuels and chemicals, A. Hollaender, ed. p. 111, New York: Plenum Press.
Rouau, X. and Odier, E. (1986).Carbohyd. Research 145, 279–292.
Uchino, F. and Nakane, T. (1981).Agric. Biol. Chem. 45, 1121–1127.
Woodward, J. (1984). Xylanases: functions, properties and applications. In:Topics in enzyme and fermentation biotechnology, A. Wiseman, ed. vol. 8, p. 9, London: Ellis Harwood Ltd.
Weber, K. and Osborn, M. (1975). Proteins and sodium dodecyl sulphate: molecular weight determination on polyacrylamide gels and related procedures. In:The proteins, H. Neurath, R.L. Hill and C. Boeder, eds. vol. 1, p. 179, New York: Academic Press.
Winter, A., Ek, K. and Anderson, U. (1977).Application note, 250, Bromma, Sweden: LKB-Produkter AB.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kelly, C.T., O'Mahony, M.R. & Fogarty, W.M. Extracellular xylanolytic enzymes ofPaecilomyces varioti . Biotechnol Lett 11, 885–890 (1989). https://doi.org/10.1007/BF01026846
Issue Date:
DOI: https://doi.org/10.1007/BF01026846