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A lipase preparation with dual pH optima, wide temperature optima and broad substrate specificity for multiple applications

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Summary

Optimised procedures for the production and recovery of lipase at 25 litre scale by submerged fermentation using aRhizopus species isolated from petroleum-soaked soil are developed. These lipase preparations are characterized by dual pH optima (4.0 and 8.0), wide temperature optima (over 80% activity between 40–57°C), broad specificity for substrates, high degree of tolerance for common salts and excellent stability during usage as well as storage.

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Author notes

  1. R. M. Rothari

    Present address: Synbiotics Limited, 390 007, Baroda, (India)

Authors and Affiliations

  1. Industrial Fermentation Division, Sarabhai Research Centre, 390 007, Baroda, (India)

    C. M. Upadhyay, P. N. Nehete & R. M. Rothari

Authors
  1. C. M. Upadhyay
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  2. P. N. Nehete
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  3. R. M. Rothari
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Upadhyay, C.M., Nehete, P.N. & Rothari, R.M. A lipase preparation with dual pH optima, wide temperature optima and broad substrate specificity for multiple applications. Biotechnol Lett 11, 793–796 (1989). https://doi.org/10.1007/BF01026099

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  • DOI: https://doi.org/10.1007/BF01026099

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