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Cyclic modulation of cross-linking interactions of microtubule-associated protein-2 with actin and microtubules by protein kinase FA

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Abstract

The ATP.Mg-dependent type-1 protein phosphatase activating factor (factor FA) was identified as a brain protein kinase that could phosphorylate microtubule-associated protein-2 (MAP-2) and thereby inhibit cross-linking interactions of MAP-2 with actin filaments and microtubules isolated from porcine brain. The phosphorylation sites were found to be equally located on both projection and microtubule-binding domains of MAP-2. Phosphoamino acid analysis revealed that the phosphorylation sites were on both serine and threonine residues, indicating that factor FA is a serine/threonine-specific MAP-2 kinase. Conversely, factor FA was further identified as a MAP-2 phosphatase activator that could promote the dephosphorylation of32P-MAP-2 phosphorylated by factor FA itself and thereby potentiate cross-linking interactions of MAP-2 with actin and microtubules. Furthermore, the two opposing functions of factor FA can be selectively modulated in a reciprocal manner bypH change. For instance, alkalinepH could stimulate factor FA to work as a MAP-2 kinase but simultaneously block it to work as a MAP-2 phosphatase activator to potentiate the inhibition on the cross-linking interactions of MAP-2 with actin and microtubules. Taken together, the results provide initial evidence that a cyclic modulation of cross-linking interactions of MAP-2 with actin filaments and microtubules can be controlled by factor FA, representing an efficient cyclic cascade control mechanism for rapid structural and functional regulation of neuronal cytoskeletal system.

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Authors and Affiliations

  1. Institute of Biomedical and Life Sciences, National Tsing Hua University, Hsinchu, Taiwan, ROC

    Shiaw-Der Yang, Jen-Shin Song, Hui-Wen Liu & Wen-Hsiung Chan

  2. Institute of Molecular Cell Biology, Chang Gung Medical College, Tao-Yuan, Taiwan, ROC

    Shiaw-Der Yang, Jen-Shin Song, Hui-Wen Liu & Wen-Hsiung Chan

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  1. Shiaw-Der Yang
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Yang, SD., Song, JS., Liu, HW. et al. Cyclic modulation of cross-linking interactions of microtubule-associated protein-2 with actin and microtubules by protein kinase FA. J Protein Chem 12, 393–402 (1993). https://doi.org/10.1007/BF01025039

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