Abstract
The precursor protein honey bee prepromelittin has been expressed as a fusion protein inEscherichia coli joined to the C-terminus of a truncated form of the bacteriophage gene 10 protein via an engineered recognition sequence for Factor Xa. Factor Xa was found to cleave poorly at the engineered site, giving a low yield of the required prepromelittin. In contrast, cleavage on the C-terminal side of the sequence VLGR at residue 67 in the gene 10 sequence proceeded in high yield. Factor Xa may be inhibited by adjacent hydrophobic sequences on the C-terminal side of a potential cleavage site.
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He, M., Jin, L. & Austen, B. Specificity of Factor Xa in the cleavage of fusion proteins. J Protein Chem 12, 1–5 (1993). https://doi.org/10.1007/BF01024906
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DOI: https://doi.org/10.1007/BF01024906