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Callus and suspension cultures for biomass production ofCynara cardunculus (Compositae)

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Summary

Friable calli, obtained from hypocotyl and leaf segments ofCynara cardunculus, were used for the production of cell suspension cultures in liquid Gamborg B5 medium supplemented as for callus obtention. The low inoculum cell suspension cultures (6.9×104 cells.ml−1) presented a td=136.8 hours while those obtained with a high inoculum (15.6×104 cells.ml−1) reached a td=33.6 hours.

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Authors and Affiliations

  1. Departamento de Biologia Vegetal, Faculdade de Ciências de Lisboa, 1294, Lisboa Codex

    A. C. Figueiredo, P. Fevereiro & M. Salomé

  2. Laboratório de Engenharia Bioquímica, Instituto Superior Técnico, Av. Rovisco Pais, 1000, Lisboa

    J. M. S. Cabral & J. M. Novais

Authors
  1. A. C. Figueiredo
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  2. P. Fevereiro
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  3. J. M. S. Cabral
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  4. J. M. Novais
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  5. M. Salomé
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  6. S. Pais
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Figueiredo, A.C., Fevereiro, P., Cabral, J.M.S. et al. Callus and suspension cultures for biomass production ofCynara cardunculus (Compositae). Biotechnol Lett 9, 213–218 (1987). https://doi.org/10.1007/BF01024569

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