Summary
A method is described for calculating local myoglobin concentration in hemoglobin-free perfused guinea-pig heart by measuring the decrease of tissueP o2 during stop of perfusion. The myoglobin concentration in ca. 10 nanogram of tissue varies between 0.106 and 0.676 g-% of the wet weight. The mean value of 0.373 g-% (standard deviation of ±0.138) agrees very well with the values obtained with chemical methods by other authors. The great variation of our values is interpreted as an inhomogeneous distribution of the local myoglobin concentration.
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Part of the results have been reported during the Workshop on Oxygen Transport in Tissue, July 19–22, 1971 in Dortmund and at the “39. Tagung der Deutschen Physiologischen Gesellschaft” April 12–13, 1972 in Erlangen.
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Leniger-Follert, E., Lübbers, D.W. Determination of local myoglobin concentration in the guinea pig heart. Pflugers Arch. 341, 271–280 (1973). https://doi.org/10.1007/BF01023668
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DOI: https://doi.org/10.1007/BF01023668