Summary
Glutamine synthetase I activity ofStreptomyces coelicolor was strongly repressed by ammonia and was induced 56.8 fold in a nitrogen-free medium. Glutamine synthetase II activity was not induced even by a long-term nitrogen starvation. Therefore, glutamine synthetase I is the only active enzyme ofStreptomyces coelicolor.
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Behrmann, I., Hillemann, D., Puhler, A., Strauch, E., and Wohlleben, W. (1990).J. Bacteriol. 172, 5326–5334.
Brana, A. F., Paiva, N and Demain, A. L. (1986).J. Gen. Microbiol. 132, 84–88.
Edmands, J., Noridge, N. A. and Benson, D. R. (1987).Proc. Natl. Acad. Sci. U.S.A. 84, 6126–6130.
Fisher, S. H. and Wray, L. V. Jr. (1989) J. Bacteriol. 171, 2378–2383.
Hillemann, D., Dammann, T., Hillemann, A. and Wohlleben, W. (1993).J. Gen. Microbiol. 139, 1773–1783.
Hopwood, D.A., Bibb, M.J., Chater, K.F., Kiesser, T., Bruton, C.J., Kieser, H.M., Lydiate, D.J., Smith, C.P., Ward, J.M. and Schrempf, H. (1985). Genetic Manipulation ofStreptomyces: a Laboratory Manual. Norwich: John Innes Foundation Norwich.
Kumada, Y., Takano, E., Nagaoka, K., and Thompson, C. J. (1990).J. Bacteriol 172, 5343–5351
Paress, P. S. and Streicher, S. L. (1985).J. Gen. Microbiol. 131, 1903–1910.
Shapiro, S. (1989). Nitrogen assimilation in actinomycetes and the influence of nitrogen nutrition on actinomycete secondary metabolism. InRegulation of Secondary Metabolism in Actinomycetes, pp. 135–211. Shapiro, S. ed, Florida: CRC Boca Raton.
Shapiro, B. M. and Stadtman, E. R. (1970).Methods in Enzymol. 17A, 910–941.
Tyler, B (1978). (1978).Ann. Rev. Biochem. 47, 1127–1162.
Woods, D. R. and Reid, S. J. (1993).FEMS Microbiol. Rev. 11, 273–284.
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Nguyen, K.t., Nguyen, L.t. & Běhal, V. What type of glutamine synthetase is important forStreptomyces coelicolor A3(2) under nitrogen-limited growth conditions?. Biotechnol Lett 16, 1027–1030 (1994). https://doi.org/10.1007/BF01022397
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DOI: https://doi.org/10.1007/BF01022397