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Penicillin G acylase fromKluyvera citrophila new choice as industrial enzyme

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Summary

We propose a new and integrated method for the evaluation of industrial enzymes. The application of this method to the enzyme penicillin G acylase fromKlyvera citrophila shows very interesting industrial propects. This acylase presents a much better stability agains heat, pH or organic cosovents as compared with the more popular enzyme fromEscherichia coli. In addition, this enzyme is very easy to immobilize through its amine groups and to stabilize through multipoint covalent attachment on activated pre-existing supports.

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Authors and Affiliations

  1. Instituto de Catalisis, C.S.I.C. Universidad Autónoma, Cantobanco, 28049, Madrid, Spain

    G. Alvaro, R. Fernandez-Lafuente, C. M. Rosell, R. M. Blanco & J. M. Guisan

  2. Centro de Investigaciones Biológicas, C.S.I.C., Velazquez 144, 28006, Madrid, Spain

    J. L. Garcia-Lopez

Authors
  1. G. Alvaro
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  2. R. Fernandez-Lafuente
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  3. C. M. Rosell
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  4. R. M. Blanco
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  5. J. L. Garcia-Lopez
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  6. J. M. Guisan
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Alvaro, G., Fernandez-Lafuente, R., Rosell, C.M. et al. Penicillin G acylase fromKluyvera citrophila new choice as industrial enzyme. Biotechnol Lett 14, 285–290 (1992). https://doi.org/10.1007/BF01022325

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  • DOI: https://doi.org/10.1007/BF01022325

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