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Effect of growth rate on stability and gene expression of a recombinant plasmid during continuous culture ofEscherichia coli in a non-selective medium

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Summary

Experimental results were obtained withEscherichia coli C600 galK (GAPDH), a genetically engineered strain that synthetizes a large quantity of glyceraldehyde-3-phosphate dehydrogenase (GAPDH), (80 % of the soluble proteins). Data concerning the stability of plasmid-containing cells and gene expression as a function of dilution rate have been obtained in continuous cultures. Contrary to other studies, our results show a clear indication that the rate of the recombinant activity was dependent on dilution rate. The results support the finding that the apparent stability of the plasmid decreases with dilution rate.

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Authors and Affiliations

  1. CNRS-ENSAIA, avenue de la forêt de Haye, BP 3, 54501, Vandoeuvre-les-Nancy, France

    Nabil Nancib & Joseph Boudrant

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  1. Nabil Nancib
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  2. Joseph Boudrant
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Nancib, N., Boudrant, J. Effect of growth rate on stability and gene expression of a recombinant plasmid during continuous culture ofEscherichia coli in a non-selective medium. Biotechnol Lett 14, 643–648 (1992). https://doi.org/10.1007/BF01021635

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