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Enantioselective oxidation of mandelic acid using a phenylmalonate metabolizing pathway of a soil bacteriumAlcaligenes bronchisepticus KU 1201

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Summary

The metabolic pathway of phenylmalonic acid byAlcaligenes bronchisepticus KU1201, which catalyzes the asymmetric decarboxylation of α-aryl-α-methylmalonic acids to α-arylpropionic acids, is demonstrated. Enantioselective oxidation of mandelic acid was investigated using the metabolizing pathway in this strain. Incubation of (±)-mandelic acid withA. bronchisepticus afforded optically pure (R)-one accompanied by benzoylformic acid and benzoic acid. This enzymatic oxidation is also applicable to various substituted mandelic acids.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Science and Technology, Keio University, Hiyoshi 3-14-1, Kohoku-ku, 223, Yokohama, Japan

    Kenji Miyamoto & Hiromichi Ohta

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  1. Kenji Miyamoto
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  2. Hiromichi Ohta
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Miyamoto, K., Ohta, H. Enantioselective oxidation of mandelic acid using a phenylmalonate metabolizing pathway of a soil bacteriumAlcaligenes bronchisepticus KU 1201. Biotechnol Lett 14, 363–366 (1992). https://doi.org/10.1007/BF01021248

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  • DOI: https://doi.org/10.1007/BF01021248

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