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Effect of 5-fluoro-deoxycytidine, 5-fluoro-uracil and cycloheximide on the incorporation of14C-glucose and32P into different cell components of rat embryos in vivo

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References

  • Bennett, L. L., Smithers, D., Ward, C. T.: Inhibition of DNA synthesis in mammalian cells by aotidione (=cycloheximide). Biochim. biophys. Acta (Amst.)87, 60–69 (1964).

    Google Scholar 

  • Bosch, L., Harbers, E., Heidelberger, C.: Studies on fluorinated pyrimidines. V Effects on nucleic acid metabolism in vivo. Cancer Res.18, 335–343 (1958).

    Google Scholar 

  • Burton, K.: A study of the conditions and mechanism of the reaction for the colorimetric estimation of deoxyribunucleic acid. Biochem. J.62, 315–323 (1956).

    Google Scholar 

  • Chaudhuri, N. K., Montag, B. J., Heidelberger, C.: Studies on fluorinated pyrimidines III. The metabolism of 5-fluorouracil-2-C14- and 5-fluororotic-2-C14 acid in vivo. Cancer Res.18, 318–328 (1958).

    Google Scholar 

  • Cheong, L., Rich, M. A., Eidinoff, M. L.: Mechanism of growth inhibition of HEp 1 cells by 5-fluorodeoxycytidine and 5-fluorodeoxyuridine. Cancer Res.20, 1602–1607 (1960).

    Google Scholar 

  • Danneberg, P. B., Montag, B. J., Heidelberger, C.: Studies on fluorinated pyrimidines. IV Effects on nucleic acid metabolism in vivo. Cancer Res.17, 329–334 (1958).

    Google Scholar 

  • Degenhardt, K.-H., Fränz, J., Yamamura, H.: A model in comparative teratogenesis: dose response to 5-fluoro-2′-deoxycytidine (FCdr Ro 5-1090) in organogenesis of mice of strains C 57 BL/6 H Jan Ffm and C 57 BL/10 J. Ffm. Teratology1, 311–334 (1968).

    Google Scholar 

  • Eidinoff, M. L., Knoll, J. E., Klein, D.: Effect of 5-fluorouracil on the incorporation of precursors into nucleic acid pyrimidines. Arch. Biochem.71, 274–279 (1957).

    Google Scholar 

  • Ennis, H. L., Lubin, M.: Cycloheximide: Aspects of inhibition of protein synthesis in mammalian cells. Science146, 1474–1476 (1964).

    Google Scholar 

  • Krowke, R., Neubert, D.:14C-Glucose as a Precursor of Nucleic Acids for Studying Embryotoxic Effects in vivo. In: Abstracts of the Fourth International Congress on Pharmacology, Basel 1969.

  • Pielsticker, K., Siebert, G.: A method for studying the incorporation of radioactively labelled precursors into mammalian main cell components. To be published in this journal (1971).

  • Mukherjee, K. L., Heidelberger, C.: Studies on fluorinated pyrimidines. XV Inhibition of incorporation of formate -C14 into DNA thymine of Ehrlich ascites carcinoma cells by 5-fluoro-2′-deoxyuridine-5′-monophosphate and related components. Cancer Res.22, 815–822 (1962).

    Google Scholar 

  • Murphy, M. L.: Factors influencing teratogenic response to drugs. Edited by Wilson, J. G., Warkany, J.: Teratology Principles and Techniques, Chapter 7. Chicago: The University of Chicago Press 1965.

    Google Scholar 

  • Sköld, O.: Enzymic ribosidation and ribosidation of 5-fluorouracil by extracts of the Ehrlich ascites tumor. Biochim. biophys. Acta (Amst.)29, 651 (1958).

    Google Scholar 

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This work was generously supported by grants from the Deutsche Forschungsgemeinschaft given to the Sonderforschungsbereich 29, Embryonal-Pharmakologie.

We are greatly indebted to Dr. Hennes and J. Gaidas from Fa. Hoffmann-La Roche, Grenzach, for generously supplying us with PCdr and FU.

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Krowke, R., Siebert, G. & Neubert, D. Effect of 5-fluoro-deoxycytidine, 5-fluoro-uracil and cycloheximide on the incorporation of14C-glucose and32P into different cell components of rat embryos in vivo. Naunyn-Schmiedebergs Arch. Pharmak. 268, 229–234 (1971). https://doi.org/10.1007/BF01020077

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  • DOI: https://doi.org/10.1007/BF01020077

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