Synopsis
Alkaline nucleoside diphosphatase, myosin ATPase and possibly an isozyme of the second, have been demonstrated histochemically at pH 9.4 in the arteriole smooth muscle cells and juxtaglomerular cells of the normal rat kidney.
False negative results and non-specific reactions for calcium-activated ATPase have been prevented, at both the optical and the electron microscopical level, by adding cysteine to the incubating medium.
Neither juxtaglomerular cells nor arteriole smooth muscle cells exhibited any histochemically demonstrable magnesium-activated ATPase, alkaline phosphatase or 5′-nucleosidase.
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References
Adams, C. W. M. (1967).Vascular histochemistry, pp. 271–4. London: Lloyd-Luke.
Brooke, H. M. &Kaiser, H. H. (1969). Some comments on the histochemical characterisation of muscle adenosine triphosphatase.J. Histochem. Cytochem. 17, 431–2.
Burt, V. K. &Green, J. W. (1971). Studies of a calcium-sensitive ATPase in chick heart ventricle cells.Expl Cell Res. 65, 170–6.
Cameron, C. H. S. (1970). Electron histochemistry of normal and dystrophic muscle.J. med. Lab. Technol. 27, 171–89.
Drews, G. A. &Engel, W. K. (1966). Reversal of the ATPase reaction in muscle fibres by EDTA.Nature, Lond. 212, 1551–3.
Gomba, SZ., Soltesz, B. M. &Szokoly, V. (1967). Studies on the histochemistry of phosphatase enzymes in the juxtaglomerular complex.Histochemie 8, 264–74.
Hayashi, M. &Freiman, D. G. (1966). An improved method of fixation for formaline sensitive enzymes with special reference to myosin adenosine triphosphatase.J. Histochem. Cytochem. 14, 577–81.
Hess, R. &Pearse, A. G. E. (1961). Mitochondrial glycerophosphate dehydrogenase activity of juxtaglomerular cells in experimental hypertension and adrenal insufficiency.Proc. Soc. exp. Biol. Med. 106, 895–8.
Meijer, A. E. F. H. (1970). Histochemical methods for the demonstration of myosine adenosine triphosphatase in muscle tissue.Histochemie 22, 51–8.
Padykula, H. A. &Herman, E. (1955). Factors affecting the activity of adenosine triphosphatase and other phosphatases as measured by histochemical technique.J. Histochem. Cytochem. 3, 161–9.
Pearse, A. G. E. (1968).Histochemistry: theoretical and applied, Vol. 1, 3rd Ed. London: Churchill.
Peart, S. W. (1969). The renin-angiotensin system—A history and review of the renin-angiotensin system.Proc. Roy. Soc. B. 174, 317–25.
Tunik, B. D. (1971). Cytochemical localization of myofibrillar adenosine triphosphatase activity in sarcomeres of glycerinated muscle by the calcium precipitation method. A novel control is needed to demonstrate artifacts.J. Histochem. Cytochem. 19, 75–84.
Van Breda Vriesman, P. J. C. (1968).Canine renal homografts. Histopathological, histochemical and biochemical studies. Thesis, University of Lieden.
Vye, M. V., Fishman, D. A. &Hansen, J. L. (1969). Ultrastructural localization of adenosine triphosphatase activity in skeletal muscle by calcium precipitation at high pH.Virchows Arch. path. Abt. B. 3, 307–23.
Wachstein, M. &Meisel, E. (1957). Histochemistry of hepatic phosphatases at a physiological pH with special reference to the demonstration of bile canaliculi.Am. J. Clin. Path. 27, 13–23.
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Barbolini, G., Kolendo, A.B. & Pearse, A.G.E. Adenosine triphosphatases and related enzymes of the juxtaglomerular and arteriole smooth muscle cells in rat kidney. Histochem J 3, 435–443 (1971). https://doi.org/10.1007/BF01014781
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DOI: https://doi.org/10.1007/BF01014781