Zusammenfassung
Bei 25 Patienten mit chronisch-lymphatischer LeukÄmie (CLL) und 23 Kontrollpersonen wurden die AktivitÄten der Purin-Interconversionsenzyme Adenosin Deaminase (ADA), Adenosin Kinase (AK), AdeninPhosphoribosyltransferase (APRT), Hypoxanthin-Guanin-Phosphoribosyl-transferase (HGPRT) und Purin-Nucleosid-Phosphorylase (PNP) bestimmt. Dabei konnte bei den Patienten mit CLL eine statistisch signifikant verminderte PNP-AktivitÄt sowie eine grenzwertig reduzierte ADA-AktivitÄt festgestellt werden, wÄhrend die AktivitÄten der anderen untersuchten Purin-Interconversionsenzyme im Normalbereich lagen.
Summary
Activities of adenosine deaminase (ADA), adenosine kinase (AK), adenine phosphoribosyltransferase (APRT), hypoxanthine guanine phosphoribosyltransferase (HGPRT), and purine nucleoside phosphorylase (PNP), all enzymes of the purine interconversion system, were determined in lymphocytes of 25 patients with chronic lymphatic leukemia (CLL) and in 23 controls. A statistically significant decrease of PNP activities and a reduction of ADA activities at borderline levels were found in the patients, whereas for the other enzymes assayed no deviation from normal values was observed.
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References
Allison, A.C., Hovi, T., Watts, R.W.E., Webster, A.D.B.: Purine and Pyrimidine Metabolism. The role of de novo purine synthesis in lymphocyte transformation. Ciba Foundation Symposium. 48, p. 207. North Holland, Amsterdam: Elsevier, Excerpta Medica 1977
Argawal, R.P., Crabtree, G.W., Parks, R.L., Nelson, J.A., Keightley, R., Parkman, R., Rosen, R. S., Stern, R. C., Polmar, S. H.: Purine nucleotide metabolism in the erythrocytes and severe combined immunodeficiency. J. Clin. Invest.57, 1025 (1976)
Coleman, M.S., Donofrio, J., Hutton, J. J., Hahn, L., Daoud, A., Lampkin, B., Dyminski, J.: Identification and quantitation of adenine deoxynucleotides in erythrocytes of a patient with adenosine deaminase deficiency and severe combined immunodeficiency. J. Biol. Chem.253, 1619 (1978)
Dietz, A.A., Virginia Czebotar: Purine metabolic cycle in normal and leukemic leukocytes. Cancer Res.37, 418 (1977)
Giblett, E.R., Anderson, J.E., Cohen, F., Pollara, B., Meuwissen, H. J.: Adenosine-deaminase deficiency in two patients with severely impaired cellular immunity. LancetII, 1067 (1972)
Grenn, H., Chan, T. S.: Pyrimidine starvation by adenosine in fibroblasts and lymphoid cells: role of adenosine deaminase. Science182, 836 (1973)
Hershfield, M.S., Snyder, F.F., Seegmiller, J.E.: Adenine and adenosine are toxic to human lymphoblasts. Mutants defective in purine salvage enzymes. Science197, 1284 (1977)
Ito, K., Uchino, H.: Control of pyrimidine biosynthesis in human lymphocytes. J. Biol. Chem.251, 1427 (1976)
Kelley, W.N., Rosenbloom, F.M., Henderson, J.F., Seegmiller, J.E.: A specific enzyme defect in gout associated with overproduction of uric acid. Proc. Natl. Acad Sci. USA57, 1735 (1967)
Meier, J., Colemann, M.S., Hutton, J.J.: Adenosine deaminase activity in peripheral blood cells of patients with haematological malignancies. Br. J. Cancer33, 312 (1975)
Ramot, B., Brok-Simoni, F., Barnea, N., Bank, I., Holtzmann, F.: Adenosine deaminase (ADA) activity in lymphocytes of normal individuals and patients with chronic lymphatic leukemia. Br. J. Haematol.36, 66 (1977)
Rai, K.R., Sawitsky, A., Cronkite, E.P., Chanana, A.D., Levy, R.N., Pasternak, S.: Clinical staging of chronic lymphatic leukemia, Blood46, 219 (1975)
Schwartz, A.L., Stern, R.C., Polmar, St. H.: Demonstration of an adenosine receptor on human lymphocytes in vitro and its possible role in the adenosine deaminase-deficient form of severe combined immunodeficiency. Clin. Immunol. Immunopathol.9, 499 (1978)
Scholar, E. M., Calabresi, R.: Identification of the enzymatic pathways of nucleotide metabolism in human lymphocytes and leukemia cells. Cancer Res.33, 94 (1973)
Simmonds, H.A., Panayi, G.S., Corrigal, V.: A role for purine metabolism in the immune response: Adenosine-deaminase activity and deoxyadenosine catabolism. LancetI, 60 (1978)
Sullivan, J.L., Osborne, W.R., Wedgwood, R. J.: Adenosine deaminase activity in lymphocytes. Br. J. Haematol.38, 157 (1978)
Tung, R., Silber, R., Quagliata, R., Conklyn, M., Gottesman, J., Hirschhorn, R.: Adenosine deaminase activity in chronic lymphatic leukemia. J. Clin. Invest.57, 756 (1976)
Uitendaal, M.P., De Bruyn, C.H.M.M., Oei, T.L., Hösli, P.: Ultramicrochemical studies on enzyme kinetics. Adv. Exp. Med. Biol.76A, 597 (1977)
Uitendaal, M.P., De Bruyn, C.H.M.M., Oei, T.L., Hösli, P.: Characterisation of purine nucleoside phosphorylase from fibroblast using ultra-microchemical methods. Hum. Hered.28, 151 (1978)
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This work was supported in part by the Austrian Research Fund (Project No. 3796).
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Ludwig, H., Kuzmits, R., Pietschmann, H. et al. Enzymes of the purine interconversion system in chronic lymphatic leukemia: Decreased purine nucleoside phosphorylase and adenosine deaminase activity. Blut 39, 309–315 (1979). https://doi.org/10.1007/BF01014193
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DOI: https://doi.org/10.1007/BF01014193
Schlüsselwörter
- Chronisch-lymphatische LeukÄmie
- Enzyme des Purin-Interconversionssystems
- Purin-Nucleosid-Phosphorylase
- Adenosin-Deaminase