Summary
Production of phenylacetylcarbinol (PAC) was measured in various yeast species. The yeast strains tested were cultivated under submerged conditions in a medium containing corn steep and sucrose as the main components; sucrose, acetaldehyde and benzaldehyde were added to the grown cultures. In a first series of experiments the initial rate of PAC production, i.e. the PAC production determined 30 min after the addition of benzaldehyde was determined in 38 yeast strains, mostly of the generaSaccharomyces andCandida. The amount of PAC produced varied from zero (12 strains) to 1.24 mg ml−1. In a second series of experiments, 15 strains, which in the first series had shown a higher PAC production, were further tested. Sucrose, acetaldehyde and benzaldehyde were added to the cultures until the PAC production ceased. The highest PAC production (6.3 mg ml−1) was reached in the strainSaccharomyces carlsbergensis “Budvar”; the production was slightly lower in 4 strains of the generaSaccharomyces, Candida andHansenula.
Similar content being viewed by others
References
Bečvářová H, Hanč O (1963) Production of Phenylacetylcarbinol by Various Yeast Species. Folia Microbiol 8:42–47
Gröger D, Schmauder HP, Mothes K (1966) Untersuchungen über die Gewinnung von 1-Phenylacetylcarbinol. Z Allg Mikrobiol 6:275–287
Gupta KG, Singh J, Sahni G, Dhawan S (1979) Production of phenyl acetyl carbinol by yeasts. Biotechnol Bioeng 21:1085–1089
Hanč O, Kabáč B (1956) Hefecarboxylase und die Bildung von Phenylacetylcarbinol. Naturwiss 43:498
Lodder J (1970) The Yeasts. North-Holland, Amsterdam London
Neuberg C, Hirsch J (1922) Zur Klassifizierung der Carboligase. Biochem Z 128:608–609
Neuberg C, Liebermann L (1921) Zur Kenntnis der Carboligase II. Biochem Z 121:311–325
Voets JP, Vandamme EJ, Vlerick C (1973) Some aspects of the phenylacetylcarbinol biosynthesis by Saccharomyces cerevisiae. Z Allg mikrobiol 13:355–366
Vojtíšek V, Netrval J (1982) Effect of pyruvate decarboxylase activitity and of pyruvate concentration on the production of 1-hydroxy-l-phenylpropanone inSaccharomyces carlsbergensis. Folia Microbiol 27:173–177
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Netrval, J., Vojtíšek, V. Production of phenylacetylcarbinol in various yeast species. European J. Appl. Microbiol. Biotechnol. 16, 35–38 (1982). https://doi.org/10.1007/BF01008240
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01008240