Abstract
Three genes encoding U4 small nuclear RNA (U4 snRNA) in the higher plantArabidopsis thaliana have been isolated and characterized. Two of the genes, AtU4.1 and AtU4.2, contain all the transcriptional signals known to be essential for U-snRNA gene activity in dicot plants: the Upstream Sequence Element (USE), the -30 TATA box and the downstream 3′ end formation sequence. The USE and TATA elements are centered approximately four helical DNA turns apart, a feature characteristic of RNA polymerase II-transcribed U-snRNA genes of plants. The genes AtU4.1 and AtU4.2 are actively transcribed in transfected plant protoplasts and inArabidopsis plants. Expression of the third gene, AtU4.3, could not be demonstrated. Since this gene is missing the downstream signal important for RNA 3′ end formation, it probably represents a pseudogene. The genes AtU4.1 and AtU4.2 encode 152–153 nt long RNAs which show 85–89% sequence similarity with broad bean and pea U4 RNAs and 60–65% similarity with mammalian U4 RNAs.Arabidopsis U4 and U6 snRNAs can be folded into the base-paired Y-shaped model supporting the importance of the U4/U6 interaction during pre-mRNA splicing in plants as well as animals.
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References
Reddy R & Busch H (1988) In: Birnstiel ML (Ed) Structure and Function of Major and Minor Small Nuclear Ribonucleoprotein Particles (pp 1–37) Springer Verlag, New York
Lührmann R, Kastner B & Bach M (1990) Biochem. Biophys. Acta 1087: 265–292
Guthrie C & Patterson B (1988) Ann. Rev. Biochem. 22: 387–419
Green MR (1991) Ann. Rev. Cell Biol. 7: 213–218
Brow DA & Guthrie C (1988) Nature 334: 213–218
Brow DA & Guthrie C (1989) Nature 337: 14–15
Dahlberg JE & Lund E (1988) In: Birnstiel ML (Ed) Structure and Function of Major and Minor Small Nuclear RNAs (pp 38–70) Springer-Verlag, Heidelberg
Kunkel GR (1991) Biochem. Biophys. Acta. 1088: 1–9
Goodall GK, Kiss T & Filipowicz W (1991) Oxford Surv. Plant Mol. Cell Biol. 7: 255–296
Sing R & Reddy R (1989) Proc. Natl. Acad. Sci. U.S.A. 86: 8280–8283
Waibel F & Filipowicz W (1990) Nature 346: 199–202
Waibel F & Filipowicz W (1990) Nucl. Acids. Res. 18: 3451–3458
Vankan P & Filipowicz W (1989) EMBO J. 8: 3875–3882
Kiss T, Marshallsay C & Filipowicz W (1991) Cell 65: 517–526
Vankan P & Filipowicz W (1988) EMBO J. 7: 791–799
Vankan P, Edoh D & Filipowicz W (1989) Nucl. Acids. Res. 16: 10425–10440
Abel S, Kiss T & Solymosy F (1989) Nucl. Acids. Res. 17: 6319–6337
van Santen VL, Swain W & Spritz RA (1988) Nucl. Acids. Res. 16: 5176
Hanley BA & Schuler MA (1989) Nucl. Acids. Res. 17: 10106
Brown JWS & Waugh R (1989) Nucl. Acids. Res. 17: 8991–9001
Waugh R, Clark G, Vaux P & Brown JWS (1991) Nucl. Acids. Res. 19: 249–256
Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning A Laboratory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Kiss T, Jakab G, Antal M, Palfi Z, Hegyi H, Kis M & Solymosy F (1988) Nucl. Acids. Res. 16: 5407–5426
Goodall GJ, Wiebauer K & Filipowicz W (1990) Meth. Enzym. 181: 148–161
Hanley BA & Schuler MA (1991) Nucl. Acids Res. 19: 1861–1868
Marshallsay C, Kiss T & Filipowicz W (1990) Nucl. Acids. Res. 18: 5280
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Hofmann, C.J.B., Marshallsay, C., Waibel, F. et al. Characterization of the genes encoding U4 small nuclear RNAs inArabidopsis thaliana . Mol Biol Rep 17, 21–28 (1993). https://doi.org/10.1007/BF01006396
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DOI: https://doi.org/10.1007/BF01006396