Skip to main content
SpringerLink
Log in

A comparison of tinctorial and immunohistological methods for the detection of fibrinoid change and fibrin deposition in the kidney

  • Papers
  • Published:
The Histochemical Journal Aims and scope Submit manuscript

Synopsis

Renal tissue from six cases with fibrinoid change or with glomerular ‘fibrin’ deposits was examined by the standard tinctorial methods (Lendrum's Martius Scarlet Blue, phosphotungsticacid-Haematoxylin and Lendrum's picro-Mallory) for the demonstration of fibrin. It was found that all three methods should be used to detect its presence. Examination by immunofluorescence techniques indicated that little or no fibrinogen or fibrin need be present in areas giving positive tinctorial reactions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aikawa, M. &Koletsky, S. (1970). Arteriosclerosis of the mesenteric arteries of rats with renal hypertension.Am. J. Path. 61, 293–322.

    Google Scholar 

  • Baker, R. D. &Selikoff, E. (1952). The cholesterol of hyaline arteriosclerosis.Am. J. Path. 28, 573–81.

    Google Scholar 

  • Burkholder, P. M. (1965a). Immunohistopathologic study of localised plasma proteins and fixation of guinea pig complement in renal lesions of diabetic glomerulosclerosis.Diabetes 14, 755–70.

    Google Scholar 

  • Burkholder, P. M. (1965b). Malignant nephrosclerosis. An immunohistopathologic study on localized γ-globulin and fixation of guinea pig complement in human kidneys.Archs. Path. Chicago 80, 583–9.

    Google Scholar 

  • Cornog, J. L., Rawson, A. J., Karp, L. A. &Arvan, D. A. (1970). Immunofluorescent and ultrastructural study of the renal glomerulus in renal vein thrombosis.Lab. Invest. 22, 101–10.

    Google Scholar 

  • Craig, J. M. &Gitlin, D. (1957). The nature of the hyaline thrombi in thrombotic thrombocytopenic purpura.Am. J. Path. 33, 251–65.

    Google Scholar 

  • Crawford, I. &Woolf, N. (1960). Hyaline arteriolosclerosis in the spleen: An immuno-histochemical study.J. Path. Bact. 79, 221–5.

    Google Scholar 

  • Davies, M. J., Woolf, N. &Carstairs, K. C. (1966). Immunohistochemical studies in diabetic glomerulosclerosis.J. Path. Bact. 92, 441–5.

    Google Scholar 

  • Dixon, F. J., Feldman, J. D. &Vasquez, J. J. (1961). Experimental glomerulonephritis: The pathogenesis of a laboratory model resembling the spectrum of human glomerulonephritis.J. exp. Med. 113, 899–919.

    Google Scholar 

  • Duffy, J. L., Cinque, T., Grishman, E. &Churg, J. (1970). Intraglomerular fibrin, platelet aggregation and subendothelial deposits in lipoid nephrosis.J. clin. Invest. 49, 251–8.

    Google Scholar 

  • Feldman, J. D., Mardiney, M. R., Unanue, E. R. &Cutting, H. (1966). The vascular pathology of thrombotic thrombocytopenic purpura.Lab. Invest. 15, 927–46.

    Google Scholar 

  • Giese, J. (1964a). Acute hypertensive vascular disease. I. Relation between blood pressure changes and vascular lesions in different forms of acute hypertension.Acta. path. microbiol. Scand. 62, 481–96.

    Google Scholar 

  • Giese, J. (1964b). Studies on vascular reaction patterns and permeability changes by means of vital microscopy and colloidal tracer techniques.Acta. path. microbiol. Scand. 62, 497–515.

    Google Scholar 

  • Gitlin, D., Craig, J. M. &Janeway, C. A. (1957). Studies on the nature of fibrinoid in the collagen diseases.Am. J. Path. 33, 55–77.

    Google Scholar 

  • Gitlin, D. &Craig, J. M. (1957). Variations in the staining characteristics of human fibrin.Am. J. Path. 33, 267–84.

    Google Scholar 

  • Hadley, W. K. &Rosenau, W. (1967). Study of human renal disease by immunofluorescent methods.Archs. Path. Chicago 83, 342–51.

    Google Scholar 

  • Hall, C. E. (1949). Electronmicroscopy of fibrinogen and fibrin.J. biol. Chem. 179, 857–65.

    Google Scholar 

  • Haust, M. D., Wyllie, J. C. &More, R. H. (1965). Electronmicroscopy of fibrin in human atherosclerotic lesions.Exp. molec. Path. 4, 205–16.

    Google Scholar 

  • Horowitz, R. E., Burrows, L., Paronetto, F. &Wildstein, W. (1963). Immuno-cytochemical observations on canine kidney homografts.Fedn. Proc. Fedn. Am. Socs. exp. Biol. 22, 274.

    Google Scholar 

  • Huttner, I., More, R. H., Rona, G. &Jellinck, H. (1969). Diversity of fibrin ultrastructure in experimental vascular fibrinoid.Lab. Invest. 20, 588.

    Google Scholar 

  • Huttner, I., More, R. H. &Rona, G. (1970). The structural evidence of specific mechanism for increased endothelial permeability in experimental hypertension.Am. J. Path. 61, 395–404.

    Google Scholar 

  • Koss, L. G. (1952). Hyaline material with staining reaction of fibrinoid in renal lesions in diabetes mellitus.Archs. Path. Chicago 54, 528–47.

    Google Scholar 

  • Lendrum, A. C., Fraser, D. S., Slidders, W. &Henderson, R. (1962). Studies on the character and staining of fibrin.J. Clin. Path. 15, 401–13.

    Google Scholar 

  • Mallory, F. B. (1938).Pathological Technique. Philadelphia: W. B. Saunders Co.

    Google Scholar 

  • Mellors, R. C. &Ortega, L. G. (1956). Analytical pathology. III—New observations on the pathogenesis of glomerulonephritis, lipid nephrosis, periateritis nodosa, and secondary amyloidosis in man.Am. J. Path. 32, 455–99.

    Google Scholar 

  • Mellors, R. C., Ortega, L. G. &Holman, H. R. (1957). Role of gamma globulins in pathogenesis of renal lesions in systemic lupus erythematosus and chronic membranous glomerulonephritis, with an observation on the lupus erythematosis cell reaction.J. Exp Med. 106, 191–202.

    Google Scholar 

  • Moe, N. &Abildgaard, U. (1969). Histological staining properties of in vitro formed fibrin clots and precipitated fibrinogen.Acta path. microbiol. Scand. 76, 61–73.

    Google Scholar 

  • Nairn, R. C. (1969).Fluorescent Protein Tracing. Edinburgh and London: E. & S. Livingstone Ltd.

    Google Scholar 

  • Pagel, W. (1951). Polyarteritis nodosa and the ‘rheumatic’ diseases.J. Clin. Path. 4, 137–57.

    Google Scholar 

  • Pardo, Victoriano &Aburano, A. (1966). Thrombotic microangiopathy and malignant hypertension.Am. J. clin. Path. 15, 605–13.

    Google Scholar 

  • Paronetto, F. (1965). Immunocytochemical observations on the vascular necrosis and renal glomerular lesions of malignant nephrosclerosis.Am. J. Path. 46, 901–15.

    Google Scholar 

  • Pearse, A. G. E. (1968).Histochemistry: Theoretical and Applied. 3rd Ed., Vol. I. London: Churchill.

    Google Scholar 

  • Porter, K. A. (1967). Rejection in treated renal allografts.J. clin. Path. 20, 518–34.

    Google Scholar 

  • Prose, P. H., Lee, L. &Balk, S. D. (1965). Electronmicroscopic study of the phagocytic Fibrin clearing mechanism.Am. J. Path. 47, 403–17.

    Google Scholar 

  • Still, W. J. S. &Boult, E. H. (1957). Electron microscopic appearance of fibrin in thin sections.Nature 179, 868–9.

    Google Scholar 

  • Vasquez, J. J. &Dixon, F. J. (1957). Immnohistochemical study of lesions in rheumatic fever, systemic lupus erythematosus and rheumatoid arthritis.Lab. Invest. 6, 205–17.

    Google Scholar 

  • Vasquez, J. J. &Dixon, F. J. (1958). Immunohistochemical analysis of lesions associated with ‘fibrinoid change’.Archs. Path. Chicago 66, 504–17.

    Google Scholar 

  • Wagner, B. M. (1967). InThe Connective Tissue, ed. B. M. Wagner & D. E. Smith, p. 78. International Academy of Pathology Monograph. Baltimore: Williams Wilkins.

    Google Scholar 

  • Walton, K. W. (1969). Studies on the Pathogenesis of Atherosclerosis.Proc. R. Soc. Med. 62, 368–70.

    Google Scholar 

  • Walton, K. W., Williamson, N. &Johnson, A. G. (1970). The pathogenesis of atherosclerosis of the mitral and aortic valves.J. Path. 101, 205–20.

    Google Scholar 

  • Zollinger, H. V. (1950). Zur Pathogenese und pathologischen Anatomie der Hypertonie.Schweiz Med. Wschr. 80, 533–7.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Gibson Laboratories, The Radcliffe Infirmary, Oxford

    A. G. MacIver

Authors
  1. A. G. MacIver
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

MacIver, A.G. A comparison of tinctorial and immunohistological methods for the detection of fibrinoid change and fibrin deposition in the kidney. Histochem J 4, 169–176 (1972). https://doi.org/10.1007/BF01004975

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01004975

Keywords

Search

Navigation