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An electronic device for the automatic correction of fluorescence emission spectra

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Synopsis

An electronic device is described for the automatic correction of fluorescence emission spectra obtained by digital microspectrofluorometry based on multichannel scaling and single photon detection as described previously. This device consists of: (a) an arithmetic unit for the correction of the spectral values and for curve integration processes; (b) a circuit that operates directly on data in the memory of the multi-channel analyser by subtracting from them a pre-established value corresponding to the back-ground; and (c) an averaging unit for calculating a mean for the spectral value.

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References

  • Caspersson, T. (1973). Fluorometric recognition of chromosomes and chromosome regions. In:Fluorescence Techniques in Cell Biology (eds. A. A. Thaer & M. Sernetz), pp. 107–23. Berlin, Heidelberg, New York: Springer-Verlag.

    Google Scholar 

  • Cova, S., Bertolaccini, M. &Bussolati, C. (1973). The measurement of luminescence waveforms by single photon techniques.Phys. stat. sol. 18, 11–62.

    Google Scholar 

  • Cova, S., Prenna, G. & Mazzini, G. (1972). Microspectrofluorometry by single photon multiscaling.Proceedings of 4th International Congress of Histochemistry and Cytochemistry, pp. 161–2. Kyoto.

  • Cova, S., Prenna, G. &Mazzini, G. (1974). Digital microspectrofluorometry by multichannel scaling and single photon detection.Histochem. J. 6, 279–99.

    Google Scholar 

  • Cova, S., Prenna, G., Sacchi, C. A. &Svelto, O. (1974). Studies on cellular fluorescences excited by a nanosecond-pulsed tunable laser. In:Excited States of Biological Molecules (ed. J. Birks), in press. New York: Interscience.

    Google Scholar 

  • Pearse, A. G. E. &Rost, F. W. D. (1969). A microspectrofluorimeter with epi-illumination and photon counting.J. Microsc. 89, 312–28.

    Google Scholar 

  • Ploem, J. S. (1967). The use of vertical illuminator with interchangeable dichroic mirrors for fluorescence microscopy with incident light.Z. wiss. Mikrosk. 68, 123–42.

    Google Scholar 

  • Prenna, G., Mazzini, G. &Cova, S. (1974). Methodological and instrumentational aspects of cytofluorometry.Histochem. J. 6, 259–78.

    Google Scholar 

  • Ruch, F. (1960). Ein Mikrospektrograph für absorptions Messungen im ultravioletten Licht.Z. wiss. Mikrosk. 64, 453–68.

    Google Scholar 

  • Sacchi, C. A., Svelto, O. &Prenna, G. (1974). Pulsed tunable lasers in cytofluorometry.Histochem. J. 6, 251–8.

    Google Scholar 

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Authors and Affiliations

  1. Centro di Studio per l'Istochimica del C.N.R., Istituto di Anatomia Comparata dell' Università, Pavia, Italy

    G. Mazzini, G. Bottiroli & G. Prenna

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  1. G. Mazzini
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  2. G. Bottiroli
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  3. G. Prenna
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Mazzini, G., Bottiroli, G. & Prenna, G. An electronic device for the automatic correction of fluorescence emission spectra. Histochem J 7, 291–297 (1975). https://doi.org/10.1007/BF01003598

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  • DOI: https://doi.org/10.1007/BF01003598

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