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Site-specific RNA crosslinking with 4-thiouridine

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Abbreviations

4-thioU:

4-thiouridine

RNP:

ribonucleoprotein

snRNA:

small nuclear RNA

snRNP:

small nuclear ribonucleoprotein

UV:

ultraviolet

References

  1. Branch AD, Benefeld BJ, Paul CP & Robertson HD (1989) Methods Enzymol. 180: 418–442

    Google Scholar 

  2. Piñol-Roma S, Adam SA, Choi YD & Dreyfuss G (1989) Methods Enzymol. 180: 410–418

    Google Scholar 

  3. Wassarman, DA (1993) Mol. Biol. Rep. 17: 143–151

    Google Scholar 

  4. Hanna MM (1989) Methods Enzymol. 180: 383–409

    Google Scholar 

  5. Moore MJ & Sharp PA (1992) Science 256: 992–997

    Google Scholar 

  6. Wyatt JR, Sontheimer EJ & Steitz JA (1992) Genes and Dev. 6: 2542–2553

    Google Scholar 

  7. Sontheimer EJ & Steitz JA (1993) Science 262: 1989–1996

    Google Scholar 

  8. Favre A (1990) In: Morrison H (Ed) Bioorganic Photochemistry: Photochemistry and the Nucleic Acids (pp 379–425) John Wiley & Sons, New York

    Google Scholar 

  9. Yaniv M, Favre A & Barrell BG (1969) Nature 223: 1331–1333

    Google Scholar 

  10. Lemaigre Dubreiul Y, Expert-Bezançon A & Favre A (1991) Nucl. Acids Res. 19: 3653–3660

    Google Scholar 

  11. Dos Santos DV, Vianna A-L, Fourrey J-L & Favre A (1993) Nucleic Acids Res. 21: 201–207

    Google Scholar 

  12. Stade K, Rinke-Appel J & Brimacombe R (1989) Nucl. Acids Res. 17: 9889–9908

    Google Scholar 

  13. Tate W, Greuer B & Brimacombe R (1990) Nucl. Acids Res. 18: 6537–6544

    Google Scholar 

  14. Rinke-Appel J, Jünke N, Stade K & Brimacombe R (1991) EMBO J. 10: 2195–2202

    Google Scholar 

  15. Ehrenberg M & Kurland CG (1988) Methods Enzymol. 164: 611–631

    Google Scholar 

  16. Moore CL & Sharp PA (1985) Cell 41: 845–855

    Google Scholar 

  17. Mowry KL & Steitz JA (1987) Mol. Cell. Biol. 7: 1663–1672

    Google Scholar 

  18. Knapp G, Beckmann JJ, Johnson PF, Fuhrman SA & Abelson J (1978) Cell 14: 221–236

    Google Scholar 

  19. Kass S, Tye K, Steitz JA & Sollner-Webb (1990) Cell 60: 897–908

    Google Scholar 

  20. Blum B & Simpson L (1992) Proc. Natl. Acad. Sci. USA 89: 11944–11948

    Google Scholar 

  21. Pyle AM (1993) Science 261: 709–714

    Google Scholar 

  22. Milligan JF & Uhlenbeck OC (1989) Methods Enzymol. 180: 51–62

    Google Scholar 

  23. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning: A Laboratory Manual (Chapter 14) Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  24. Puglisi JD & Tinoco I (1989) Methods Enzymol. 180: 304–325

    Google Scholar 

  25. Yisraeli JK & Melton DA (1989) Methods Enzymol. 180: 42–50

    Google Scholar 

  26. Martin FH & Tinoco I (1980) Nucl. Acids Res. 8: 2295–2299

    Google Scholar 

  27. Wang J-F, Downs WD & Cech TR (1993) Science 260: 504–508

    Google Scholar 

  28. Fujimoto M, Kuninaka A & Yoshino H (1974) Agr. Biol. Chem. 38: 777–783

    Google Scholar 

  29. Steitz JA (1989) Methods Enzymol. 180: 468–481

    Google Scholar 

  30. Tye K & Steitz JA (1992) Nucl. Acids Res. 20: 5375–5382

    Google Scholar 

  31. Ericson G & Wollenzien P (1988) Anal. Biochem. 174: 215–223

    Google Scholar 

  32. Boorstein WR & Craig EA (1989) Methods Enzymol. 180: 347–369

    Google Scholar 

  33. Zaug AJ, Kent JR & Cech TR (1984) Science 224: 574–578

    Google Scholar 

  34. Hausner T-P, Giglio LM & Weiner AM (1990) Genes and Dev. 4: 2146–2156

    Google Scholar 

  35. Pitulle C, Kleineidam RG, Sproat B & Krupp G (1992) Gene 112: 101–105

    Google Scholar 

  36. Moore MJ & Sharp PA (1993) Nature 365: 364–367

    Google Scholar 

  37. Burgin AB & Pace NR (1990) EMBO J. 9: 4111–4118

    Google Scholar 

  38. Hanna MM, Zhang Y, Reidling JC, Thomas MJ & Jou J (1993) Nucl. Acids Res. 21: 2073–2079

    Google Scholar 

  39. Willis MC, Hicke BJ, Uhlenbeck OC, Cech TR & Koch TH (1993) Science 262: 1255–1257

    Google Scholar 

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Sontheimer, E.J. Site-specific RNA crosslinking with 4-thiouridine. Mol Biol Rep 20, 35–44 (1994). https://doi.org/10.1007/BF00999853

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  • DOI: https://doi.org/10.1007/BF00999853

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