Abstract
The SmN protein is closely related to the constitutively expressed RNA splicing protein SmB but is expressed only in brain and heart tissue. Mice which lack expression of SmN die shortly after birth suggesting a critical role for this protein possibly in the regulation of neuronal-specific alternative splicing events. We show here however that the neuronal-specific alternative splicing of the RNAs encoding several different classes of protein proceeds normally in mice lacking SmN expression. The potential role of SmN and the reasons for the lethal effect observed in non-expressing mice are discussed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Breitbart RE, Andreadis A & Nadal-Ginard B (1987) Ann. Rev. of Biochem. 56: 467–495
Cassidy SB & Ledbetter DH (1989) Neurol. Clin. 7: 37–54
Cattanach BM, Barr JA, Evans EP, Burtenshaw M, Beechy CV, Leff SE, Brannan CL, Copeland NG, Jenkins NA & Jones J (1992) Nat. Genet. 2: 270–274
Chomczynski P & Sacchi N (1987) S Biochem. 162: 156–159
Crawford JA, Mutchler KJ, Sullivan BE, Lanigan TM, Clark MS & Russo AF (1993) J. Biol. Chem. 268: 9879–9885
Delsert CD & Rosenfeld MG (1992) J. Biol. Chem. 267: 14573–14579
Dent CL, Lillycrop KA, Estridge JK, Thomas NSB, Lillycrop KA, Estridge JK, Thomas NSB & Latchman DS (1991) Mol. Cell. Biol. 11: 3925–3930
Grimaldi K, Horn DA, Hudson LD, Terenghi G, Barton P, Polak JM & Latchman DS (1993) Devel. Biol. 156: 319–323
Horn DA & Latchman DS (1993) Mol. Brain Res. 19: 181–187
Horn DA & Latchman DS (1993) Neuroscience Letters 155: 57–60
Horn DA, Suburo A, Terenghi G, Hudson LD, Polak JM & Latchman DS (1992) Mol. Brain Res. 16: 13–19
Huntriss JD, Latchman DS & Williams DJ (1993) Nucl. Acids Res. 21: 4047–4053
Kawasaki ES (1990) Innis MA, Gelford DH, Sninsky JJ & White TJ (Eds) PCR protocols Academic Press, San Diego pp 21–27
Kozasa T, Itoh H, Tsukamoto T & Kaziro Y (1988) Proc Natl Acad, Sci, USA 85: 2081–2085
Lamond AI (1991) Current Opinion in Cell Biol. 3: 493–500
Latchman DS (1990) The New Biologist 2: 297–303
Leff SE, Brannan CI, Reed ML, Ozcelik T, Francke U, Copeland NG & Jenkins NJ (1992) Nat. Genet. 2: 259–264
Leff SE, Evans RM & Rosenfeld MG (1987) Cell 48: 517–524
Lillycrop KA & Latchman DS (1992) J. Biol. Chem. 267: 24960–24966
20.Lillycrop KA, Dent CL, Wheatley SC, Beech MN, Ninkina NN, Wood JN & Latchman DS (1991) Neuron 7: 381–390
McAllister G, Amara SG & Lerner MR (1988) Proc Natl Acad, Sci, USA 85: 5296–5300
OzcelikS T, Leff S, Robinson W, Donlon T, Lalande M, Sanjines E, Schinzel A & Francke U (1992) Nat. Genet. 2: 265–269
Pyper JM & Bolen JB (1990) Mol. Cell. Biol. 10: 2035–2040
Reyes AA, Small SJ & Akeson R (1991) Mol. Cell. Biol. 11: 1654–1661
Schmauss C, Brines ML & Lerner MR (1992) J. Biol. Chem. 267: 8521–8529
Schmauss C, McAllister G, Ohosone Y, Hardin JA & Lerner MR (1989) Nucleic Acids Res 17: 1733–1743
Sharpe NG, Williams DG, Howarth DN, Coles B & Latchman DS (1989) FEBS Letters 250: 585–590
Sharpe NG, Williams DG, Norton PM & Latchman DS (1989) FEBS Letters 243: 132–136
Van Dam A, Winkel I, Ziglstra-Baalbergen J, Smeenk R & Cuypers HT (1989) EMBO J. 8: 3853–3860
Williams DG, Stocks MR, Smith PR & Maini RN (1986) Immunol. 58: 495–500
Wirth T, Priess A, Annweiler A, Zwilling S & Oeler B (1991) Nucl. Acids Res. 19: 43–51
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huntriss, J.D., Barr, J.A., Horn, D.A. et al. Mice lackingSnrpn expression show normal regulation of neuronal alternative splicing events. Mol Biol Rep 20, 19–25 (1994). https://doi.org/10.1007/BF00999851
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00999851