Abstract
Proteasomes (prosomes) are large multiprotein complexes. They are involved in protein degradation of ubiquitin-conjugated proteins and in the generation of MHC class I peptides. We gave further evidence that they interfere within vitro protein synthesis. Proteasomes inhibit the translation of Tobacco mosaic virus RNA. Analysis of cell-free systems by sucrose gradient centrifugation revealted that they prevent the formation of 80S initiation complexes but not the early phase of initiation.
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Schmid HP, Akhayat O, Martins de Sa C, Puvion F, Köhler K & Scherrer K (1984). EMBO J 3: 29
Martins de Sa C, Grossi de Sa MF, Akhayat O, Broders, F, Scherrer K, Horsch A & Schmid HP (1986). J Mol Biol 187: 479
Tanaka K, Tamura TJ, Yoshimura T & Ichihara A (1982). The new biologist 4: 173
Schliephacke M, Kremp A, Schmid HP, Köhler K & Kull U (1991). Eur. J. Cell. Biol. 55: 114
Rivett AJ (1993). Biochem. J. 291: 1
Falkenburg PE, Haass C, Kloetzel PM, Niedel B, Kopp F, Kühn L & Dahlmann B (1988). Nature 331: 190
Kleinschmidt JA, Escher C & Wolf DH (1988). FEBS Lett. 239: 35
Pühler G, Weinkauf S, Bachmann L, Müller S, Engel A., Hegerl R. and Baumeister W (1992). EMBO J. 11: 1607–1616
Peters JM, Cejka Z, Harris JR, Kleinschmidt JA & Baumeister WJ (1993). Mol. Biol. 234: 932
Tomek W, Buri J, Vallon R & Schmid HP (1990) J. Chromatogr. 521: 221
Arrigo AP, Darlix JL, Khandjian EW, Simon M & Spahr PF (1985). EMBO J. 4: 399
Driscoll J & Goldberg AL (1990). J. Biol. Chem., 265: 4789
Eytan E, Ganoth D, Armon T & Hershko A (1989). Proc. Natl. Acad. Sci. USA 86: 7751
Ortiz-Navarrete V, Seelig A, Gernold M, Frentzel S, Kloetzel PM & Hämmerling GJ (1991). Nature 353: 662
Martinez CJ & Monaco JJ (1991). Nature 353: 664
Horsch A, Martins de Sa C, Dineva B, Spindler E & Schmid HP (1989). FEBS Lett. 246: 131
Laemmli UK (1970). Nature 227: 680
Horsch A, Köhler K, Ellward-Tschürz M & Schmid HP (1990). FEBS Lett. 269: 336
Doyle JJ & Doyle JL (1987). Phytochem. Bull. 19: 11
Tsukahara T, Tanaka K, Ogava T, Ishiura S, Funabiki, Sugita H (1989). FEBS Let. 255: 179
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Homma, S., Horsch, A., Pouch, MN. et al. Proteasomes (prosomes) inhibit the translation of Tobacco mosaic virus RNA by preventing the formation of initiation complexes. Mol Biol Rep 20, 57–61 (1994). https://doi.org/10.1007/BF00996354
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DOI: https://doi.org/10.1007/BF00996354