Abstract
A metalloprotease activity associated with myelin membrane preparations degrades myelin basic protein (MBP), generating a characteristic fragment designated peptide C (MBP 74-170). Using an immunoblotting assay, peptide C-generating activity was detected in mammalian, avian, reptilian, and amphibian brains. The activity was present in rat brain as early as postnatal day 1 and also in adult rat peripheral nerve. Immunohistochemistry with a monoclonal antibody to the purified enzyme revealed that the metalloprotease was present in oligodendrocytes of optic nerve, of both white and grey matter of spinal cord, and also in the cytoplasm of both myelinating and non-myelinating Schwann cells of peripheral nerve.
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Banik, N. L., McAlbaney, W. W., and Hogan, E. L. 1985. Calcium-stimulated proteolysis in myelin: evidence for a Ca2+-activated neutral protease associated with purified myelin of rat CNS. J. Neurochem. 45:581–588.
Chantry, A., Earl, C., Groome, N., and Glynn, P. 1988. Metalloendoprotease cleavage of 18.2 and 14.1-kilodalton basic proteins dossociating from rodent myelin membranes. J. Neurochem. 50:688–694.
Chantry, A., Gregson, N. A., and Glynn, P. 1989. A novel metalloproteinase associated with brain myelin membranes: isolation and characterisation. J. Biol. Chem. 264, 21603–21607.
Chantry, A., and Glynn, P. 1990. A novel metalloproteinase originally isolated from brain myelin is present in many tissues. Biochem. J. 268:245–248.
Dunkley, P. R. and Carnegie, P. R. 1974. Isolation of myelin basic protein. Pages 219–245.in Marks, N., and Rodnight, R., (eds) Research Methods in Neurochemistry Vol. 2, Plenum Press, New York.
Glynn, P., Chantry, A., Groome, N., and Cuzner, M. L. 1987. Basic protein dissociating from myelin membranes at physiological ionic strength and pH is cleaved into three major fragments. J. Neurochem. 48:752–759.
Groome, N., Chantry, A., Earl, C., Newcombe, J., Keen, J., Findlay, J. and Glynn, P. 1988. A new epitope on human myelin basic protein arising from cleavage by a metalloendoprotease associated with brain myelin membranes. J. Neuroimmunol. 19:77–88.
Kelero de Rosbo, N., Carnegie, P. R., and Bernard, C. A. A. 1986. Quantitative electroimmunoblotting study of the calcium-activated neutral protease in human myelin. J. Neurochem. 47:1007–1012.
Norton, W. T., and Poduslo, S. E. 1973. Myelination in rat brain: method of myelin isolation. J. Neurochem. 21:749–758.
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Special issue dedicated to Dr. Alan N. Davison
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Chantry, A., Gregson, N. & Glynn, P. Degradation of myelin basic protein by a membrane-associated metalloprotease: Neural distribution of the enzyme. Neurochem Res 17, 861–867 (1992). https://doi.org/10.1007/BF00993261
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DOI: https://doi.org/10.1007/BF00993261