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Influence of low-intensity ultraviolet radiation on extrusion of furanocoumarins to the leaf surface

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Abstract

Exposure ofRuta graveolens leaves to low intensity 366-nm radiation led to a ca. 20% increase in concentrations of the furanocoumarins psoralen, xanthotoxin and bergapten, as compared to leaves kept in darkness. Both direct and, even more, scattered UV radiation produced increases in total concentrations. Changes in the concentrations of individual coumarins were generally parallel. Extrusion to the surface was increased, especially in lower, older leaves exposed to the scattered radiation, where it exceeded the control by factors of eight or nine. It is suggested that this response could enhance shielding of leaves against penetration of UV into the cells and that irradiation, by exciting the furanocoumarins, could augment protection against potential microbial invaders.

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References

  • Adamse, P., Kendrick, R.E., andKoornneef, M. 1988. Photomorphogenetic mutants of higher plants.Photochem. Photobiol. 48:833–841.

    Google Scholar 

  • Barnby, M.A., Yamasaki, R.B. andKlocke, J.S.. 1989. Biological activity of azarachtin, three derivatives, and their ultraviolet radiation degradation products against tobacco budworm (Lepidoptera: Noctuidae) larvae.J. Econ. Entomol. 1982:58–63.

    Google Scholar 

  • Barnes, P.W., Flint, S.D. andCaldwell, M.M. 1987. Photosynthesis damage and protective pigments in plants from latitudical arctic/alpine gradient exposed to supplemental UV-B radiation in the field.Arct. Alp. Res. 19:21–27.

    Google Scholar 

  • Beier, R.C., andOertli, E.H. 1983. Psoralen and other linear furanocoumarins as phytoalexins in celery.Phytochemistry 22:2595–2597.

    Google Scholar 

  • Beier, R.C., Ivie, G.W., andOertli, E.H. 1983. Psoralens as phytoalexins in food plants of the family Umbelliferae. Significance in relation to storage and processing, pp. 295–310,in J.W. Finley and D.E. Schwass (eds.). Xenobiotics in Foods and Feeds. American Chemical Society Symposium Series No. 234. American Chemical Society, Washington, D.C.

    Google Scholar 

  • Berenbaum, M.R. 1991. Comparative processing of allelochemicals in Papilionidae (Lepidoptera).Arch. Insect Biochem. Physiol. 17:213–221.

    Google Scholar 

  • Berenbaum, M., Zangerl, A.R., andNitao, J.K. 1986. Constraints on chemical coevolution: The parsnip webworm and the wild parsnip.Evolution 40:1215–1228.

    Google Scholar 

  • Berenbaum, M., Zangerl, A.R., andLee, K. 1989. Chemical barriers to adaptation by a specialist herbivore.Oecologia 80:501–506.

    Google Scholar 

  • Berenbaum, M.R., Nitao, J.K., andZangerl, A.R. 1991. Adaptive significance of furanocoumarin diversity inPastinaca sativa.J. Chem. Ecol. 17:207–216.

    Google Scholar 

  • Biggs, R.H., Kossuth, S.V., andTeramura, A.H. 1981. Response of 19 cultivars of soybeans to ultraviolet-B irradiation.Physiol. Plant. 53:19–26.

    Google Scholar 

  • Bogenrieder, A. 1982. Action spectra for the depression of photosynthesis by UV irradiation inLactuca sativa L. andRumex alpinus L., pp. 132–139,in H. Bauer, M.M. Caldwell, M. Tevini, and R.C. Worrest (eds.). Biological Effects of UV-B Radiation. Proceedings of the workshop held in Munich-Neuherberg. Gesellschaft für Strahlen- und Umweltforschung mbH, Munich.

    Google Scholar 

  • Bondar, L.M., andPopova, O.N. 1989. Cytogenetic analysis of the effect of chronic irradiation on naturalVicia cracca L. populations.Radiobiologiya 29:310–314.

    Google Scholar 

  • Bornman, J.F. 1989. Target sites of UV-B radiation in photosynthesis of higher plants.J. Photochem. Photobiol. B. Biol. 4:145–158.

    Google Scholar 

  • Bornman, J.F. 1991. UV radiation as an environmental stress in plants.J. Photochem. Photobiol. B: Biol. 8:337–342.

    Google Scholar 

  • Bornman, J.F., andVogelmann, T.C. 1991. Effect of UV-B radiation on leaf optical properties measured with fiber optics.J. Exp. Bot. 42:547–554.

    Google Scholar 

  • Bridge, M.A., andKlarman, W.L. 1972. Soybean phytoalexin, hydroxyphaseolin, induced by ultraviolet irradiation.Phytopathology 63:606–609.

    Google Scholar 

  • Bull, D.L., Ivie, G.W., Beier, R.C., Pryer, N.W., andOertli, E.H. 1986. In vitro metabolism of linear furanocoumarin (8-methoxycoumarin, xanthotoxin) by mixed-function oxidases of larvae of black swallowtail butterfly and fall armyworm.J. Chem. Ecol. 12:885–892.

    Google Scholar 

  • Caldwell, M.M., andWarner, C.W. 1982. UV-B sensitivity of plant photosynthesis as influenced by visible irradiation, pp. 117–120,in H. Bauer, M.M. Caldwell, M. Tevini, and R.C. Worrest (eds.). Biological Effects of UV-B Radiation. Proceedings of the workshop held in Munich-Neuherberg. Gesellschaft für Strahlen- und Umweltforschung mbH, Munich.

    Google Scholar 

  • Caldwell, M.M., Teramura, A.H., andTevini, M. 1989. The changing solar ultraviolet climate and the ecological consequences for higher plants.Trends Ecol. Evol. 4:363–366.

    Google Scholar 

  • Chappell, J., andHahlbrock, K. 1984. Transcription of plant defense genes in response to UV light or fungal elicitor.Nature 311:76–78.

    Google Scholar 

  • Chaudhary, S.K., Ceska, O., Warrington, P.J., andAshwood-Smith, M.J. 1985. Increased furanocoumarin content of celery during storage.J. Agric. Food Chem. 33:1153–1157.

    Google Scholar 

  • Cohen, M.B., Berenbaum, M.R., andSchuler, M.A. 1989. Induction of cytochrome P-450-mediated detoxification of xanthotoxin in the black swallowtail.J. Chem. Ecol. 15:2347–2356.

    Google Scholar 

  • Dercks, W., Trumble, J., andWinter, C. 1990. Impact of atmospheric pollution on linear furanocoumarin content in celery.J. Chem. Ecol. 16:443–454.

    Google Scholar 

  • Douglas, C., Hoffmann, H., Schulz, W., andHahlbrock, K. 1987. Structure and elicitor- or UV-light-stimulated expression of two 4-coumarate: CoA ligase genes in parsley.EMBO J. 6:1189–1195.

    Google Scholar 

  • Dubery, I.A. 1992. Elicitation of enhanced phenylpropanoid metabolism inCitrus flavedo by gamma radiation.Phytochemistry 31:2659–2662.

    Google Scholar 

  • Hadwiger, L.A. andSchwochau, M.E. 1971. Ultraviolet light-induced formation of pisatin and phenylalanine ammonia lyase.Plant Physiol. 47:588–590.

    Google Scholar 

  • Harborne, J.B. 1985. Phenolics and plant defense.Ann. Proc. Phytochem. Soc. Eur. 25:393–408.

    Google Scholar 

  • Harborne, J.B. 1988. Introduction to Ecological Biochemistry, 3rd ed. Academic Press, London.

    Google Scholar 

  • Hermsmeier, D., Mala, E., Schulz, R., Thielmann, J., Gallaud, P., andSenger, H. 1991. Antagonistic blue- and red-light regulation of cab-gene expression during photosynthetic adaptation inScenedesmus obliquus.J. Photochem. Photobiol. B: Biol. 11:189–202.

    Google Scholar 

  • Ivie, G.W., Bull, D.L., Beier, R.C., Pryer, N.W., andOertli, E.H. 1983. Metabolic detoxification: Mechanism of insect resistance to plant psoralens.Science 221:374–376.

    Google Scholar 

  • Jagger, J. 1981. Near-UV radiation effects on microorganisms.Photochem. Photobiol. 34:761–768.

    Google Scholar 

  • Jagger, J. 1985. Solar-UV Actions on Living Cells. Praeger, New York.

    Google Scholar 

  • Klein, R.M. 1978. Plant and near-ultraviolet radiation.Bot. Rev. 44:1–127.

    Google Scholar 

  • Klocke, J.A. andKubo, I. 1991. Defense of plants through regulation of insect feeding behavior.Fla. Entomol. 74:18–23.

    Google Scholar 

  • Lee, K. andBerenbaum, M.R. 1989. Action of antioxidant enzymes and cytochrome P-450 monooxygenases in the cabbage looper in resistance to plant phytotoxins.Arch. Insect Biochem. Physiol. 10:151–162.

    Google Scholar 

  • Lee, K. andBerenbaum, M.R. 1990. Defense of parsnip webworm against phototoxic furanocoumarins: Role of antioxidant enzymes.J. Chem. Ecol. 16:2451–2460.

    Google Scholar 

  • Lee, W.G., Hodgkinson, I.J., andJohnson, P.N. 1990. A test for ultraviolet reflectance from fleshy fruits of New Zealand plant species.N.Z. J. Bot. 28:21–24.

    Google Scholar 

  • Lois, R., Dietrich, A., Hahlbrock, K. andSchulz, W. 1989. A phenylalanine ammonia-lyase gene from parsley: Structure, regulation and identification of elicitor and light responsive cis-acting elements.EMBO J. 8:1641–1648.

    Google Scholar 

  • Martin, G., Myers, D.M. andVogelmann, T.C. 1991. Characterization of plant epidermal lens effects by a surface replica technique.J. Exp. Bot. 42:581–588.

    Google Scholar 

  • McLennan, A.G. 1987. The repair of ultraviolet light-induced DNA damage in plant cells.Mutat. Res. 181:1–7.

    Google Scholar 

  • Mirecki, R.M. andTeramura, A.H. 1984. Effects of ultraviolet-B irradiation on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion.Plant Physiol. 74:475–480.

    Google Scholar 

  • Mohle, B., Heller, W. andWellman, E. 1985. UV-induced biosynthesis of quercetin 3-O-β-D-glucuronide in dill cell cultures.Phytochemistry 24:465–467.

    Google Scholar 

  • Murali, N.S. andTeramura, A.H. 1986. Effectiveness of UV-B radiation on the growth and physiology of field-grown soybean (Glycine max) modified by water stress.Photochem. Photobiol. 44:215–219.

    Google Scholar 

  • Osman, M. andMetwally, M. 1991. Influence of UV-A irradiation on growth and lipid composition inAlternaria alternata andFusarium oxysporum.Microbios 68:147–155.

    Google Scholar 

  • Pate, D.W. 1983. Possible role of ultraviolet radiation of Cannabis chemotypes.Econ. Bot. 37:396–405.

    Google Scholar 

  • Potapenko, A.Y. 1991. Mechanisms of phytodynamic effects of furocoumarins.J. Photochem. Photobiol, B: Biol., 9:1–33.

    Google Scholar 

  • Robberecht, R. andCaldwell, M.M. 1978. Leaf epidermal transmittance of ultraviolet radiation and its implications for plant sensitivity to ultraviolet induced injury.Oecologia 32:277–287.

    Google Scholar 

  • Schulze-Lefert, P., Dangl, J.L., Becker-Andre, M., Hahlbrock, K. andSchulz, W. 1989. Inducible in vivo DNA footprints define sequences necessary for UV light activation of the parsley chalcone synthase gene.EMBO J. 8:651–656.

    Google Scholar 

  • Städler, E. andBuser, H.-R. 1984. Defense chemicals in the leaf surface wax synergistically stimulate oviposition by a phytophagous insect.Experientia, 40:1157–1159.

    Google Scholar 

  • Staiger, D., Kauten, H. andShell, J. 1989. A CACGTG motif of theAntirrhinum majus chalcone synthase promoter is recognized by an evolutionarily conserved nuclear protein.Proc. Natl. Acad. Sci. U.S.A. 86:6930–6934.

    Google Scholar 

  • Sullivan, J.H., andTeramura, A.H. 1988. Effects of ultraviolet-B irradiation on seedling growth in Pinaceae.Am. J. Bot. 75:225–230.

    Google Scholar 

  • Teramura, A.M. andSullivan, J.H. 1987. Soybean growth responses to enhanced levels of ultraviolet-B radiation under greenhouse conditions.Am. J. Bot. 74:975–979.

    Google Scholar 

  • Tevini, M., andTeramura, A.M. 1989. UV-B effects on terrestrial plants.Phytochem. Photobiol. 50:479–487.

    Google Scholar 

  • Thoma, V., andTevini, M. 1982. Effect of enhanced UV-B irradiation on leaf surface and anatomy, pp. 83–92,in H. Bauer, M.M. Caldwell, M. Tevini, and R.C. Worrest (eds.). Biological Effects of UV-B Radiation. Proceedings of the workshop held in Munich-Neuherberg. Gesellschaft für Strahlen- und Umweltforschung mbH, Munich.

    Google Scholar 

  • Thompson, H.J., andBrown, S.A. 1984. Separations of some coumarins of higher plants by liquid chromatography.J. Chromatogr. 314:323–336.

    Google Scholar 

  • Tietjen, K.G. andMatern, U. 1983. Different response of cultured parsley cells to elicitors from two non-pathogenic strains of fungi. 2. Effects on enzyme activities.Eur. J. Biochem. 131:409–413.

    Google Scholar 

  • Trumble, J.T., Dercks, W., Quiros, C.F., andBeier, R.C. 1990. Host plant resistance and linear furanocoumarin content ofApium accessious.J. Econ. Entomol. 83:519–525.

    Google Scholar 

  • Trumble, J.T., Moar, W.J., Brewer, M.J., andCarson, W.G. 1991. Impact of UV radiation on activity of linear furanocoumarins in theBacillus thuringiensis var. Kurstaki againstSpodoptera exigna: Implications for tritropic interactions.J. Chem. Ecol. 17:973–988.

    Google Scholar 

  • Tyrrell, R.M. 1978. Radiation synergism and antagonism.Phytochem. Phytobiol. Rev. 3:35–113.

    Google Scholar 

  • Tyrrell, R.M., andPeak, M.J. 1978. Interactions between UV radiation of different energies in the interaction of bacteria.J. Bacteriol. 136:437–440.

    Google Scholar 

  • Weber, G., Monajembaski, F., Greulich, K.O., andWolfrum, J. 1991. Genetic changes induced in higher plants by a UV laser microbeam.Isr. J. Bot. 40:115–122.

    Google Scholar 

  • Zangerl, A.R. andBerenbaum, M.R. 1987. Furanocoumarins in wild parsnip: Effect of photosynthetically active radiation, ultraviolet light and nutrients.Ecology 68:516–520.

    Google Scholar 

  • Zangerl, A.R., andBerenbaum, M.R. 1990. Furanocoumarin induction in wild parsnip: Genetics and populational variation.Ecology 71:1933–1940.

    Google Scholar 

  • Zobel, A.M. 1991. Effect of the change from field to greenhouse environment on the linear furanocoumarin levels ofRuta chalepensis.J. Chem. Ecol. 17:21–27.

    Google Scholar 

  • Zobel, A.M., andBrown, S.A. 1988. Determination of furanocoumarins on the leaf surface ofRuta graveolens with an improved extraction technique.J. Nat. Prod. 51:941–946.

    Google Scholar 

  • Zobel, A.M., andBrown, S.A. 1989. Histological localization of furanocoumarins inRuta graveolens.Can. J. Bot. 67:915–921.

    Google Scholar 

  • Zobel, A.M., andBrown, S.A. 1990. Seasonal changes of furanocoumarin concentrations in leaves ofHeracleum lanatum.J. Chem. Ecol. 16:1623–1634.

    Google Scholar 

  • Zobel, A.M., andMarch, R.E. 1993. Autofluorescence reveals different histological localizations of furanocoumarins in fruits of some Umbelliferae and Leguminosae.Ann. Bot. 70:In press.

  • Zobel, A.M., andNighswander, J.E. 1990. Accumulation of phenolic compounds in the necrotic areas of Austrian and red pine needles due to salt spray.Ann. Bot. 66:629–640.

    Google Scholar 

  • Zobel, A.M., andNighswander, J.E. 1991. Accumulation of phenolic compounds in the necrotic areas of Austrian and red pine needles due to sulphuric acid spray as a bioindicator of air pollution.New Phytol. 117:565–576.

    Google Scholar 

  • Zobel, A.M., Brown, S.A., andNighswander, J.E. 1991. Effect of simulated acid spray on furanocoumarin concentrations on the surface ofRuta graveolens leaves.Ann. Bot. 67:213–218.

    Google Scholar 

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  1. Department of Chemistry, Trent University, K9J 7B8, Peterborough, Ontario, Canada

    Alicja M. Zobel & Stewart A. Brown

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Zobel, A.M., Brown, S.A. Influence of low-intensity ultraviolet radiation on extrusion of furanocoumarins to the leaf surface. J Chem Ecol 19, 939–952 (1993). https://doi.org/10.1007/BF00992529

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