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RAPD characterisation of two neotropical hybrid legumes

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Abstract

An investigation of randomly amplified polymorphic DNA (RAPD) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) marker distribution was made for two well-characterised hybrids and their parents,Leucaena leucocephala andL. esculenta andParkinsonia aculeata andCercidium praecox. Three chloroplast DNA (cpDNA) markers identified the maternal parent of eachL. leucocephala ×L. esculenta hybrid. Fifteen species-diagnostic RAPD markers (invariant in one taxon and absent from the other) were always present in theLeucaena hybrid and assumed to be of nuclear origin, whilst three RAPD markers showed expression patterns identical to the cpDNA markers and were assumed to be of organellar origin. No RAPD or PCR-RFLP taxon-diagnostic markers were discovered for eitherP. aculeata orC. praecox. However, 21 RAPD markers were species-specific (polymorphic within one taxon but absent from the other) and Southern analysis indicated that none of the markers were of organellar origin. Only 67% additivity of markers specific toP. aculeata andC. praecox was demonstrated in the hybrids between these two species, whilst inLeucaena 97% additivity was demonstrated. Differences between the two hybridising situations were related to the behaviour of the molecular markers and the biology of the species.

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References

  • Adams, R. P., 1982: A comparison of multivariate methods for the detection of hybridisation. — Taxon31: 646–661.

    Google Scholar 

  • Anderson, E., 1949: Introgressive hybridisation. — New York: Wiley.

    Google Scholar 

  • Arnold, M. L., Buckner, C. M., Robinson, J. J., 1991: Pollen-mediated introgression and hybrid speciation in Louisiana irises. — Proc. Natl. Acad. Sci. USA88: 1398–1402.

    Google Scholar 

  • Avise, J. C., 1994: Molecular markers, natural history and evolution. — New York: Chapman & Hall.

    Google Scholar 

  • , 1984: Hybridisation and introgression among species of sunfish (Lepomis): Analysis by mitochondrial DNA and allozyme markers. — Genetics108: 237–255.

    Google Scholar 

  • Backeljau, T., De Bruyn, L., De Wolf, H., Van Dongen, S., Verhagen, R., Winnepenninckx, B., 1995: Randomly amplified polymorphic DNA and parsimony methods. — Cladistics11: 119–130.

    Google Scholar 

  • Black, W. C., 1993: PCR with arbitrary primers: approach with care. — Insect Molec. Biol.2: 1–6.

    Google Scholar 

  • Carter, A. M., Rem, N. C., 1974: Pollen studies in relation to hybridisation inCercidium andParkinsonia (Leguminosae;Caesalpinioideae). — Madroño22: 301–311.

    Google Scholar 

  • Chase, M. W., Hills, H. G., 1991: Silica gel: an ideal material for field preservation of leaf samples for DNA studies. — Taxon40: 215–220.

    Google Scholar 

  • Clark, A. G., Lanigran, C. M. S., 1993: Prospects for estimating nucleotide divergence with RAPDs. — Molec. Biol. Evol.10: 1096–1111.

    Google Scholar 

  • Crawford, D. J., Brauner, S., Cosner, M. B., Stuessy, T. F., 1993: Use of RAPD markers to document the origin of the intergeneric hybrid ×Margyracaena skottsbergii (Rosaceae) on the Juan Fernandez Islands. — Amer. J. Bot.80: 89–92.

    Google Scholar 

  • Dawson, I. K., Simons, A. J., Waugh, R., Powell, W., 1996: Detection and pattern of interspecific hybridisation betweenGliricidia sepium andG. maculata in Meso-America revealed by PCR-based assays. — Molec. Ecol.5: 89–98.

    Google Scholar 

  • Dewayne Shoemaker, D., Ross, K. G., Aronold, M. L., 1994: Development of RAPD markers in two introduced fire ants,Solenopsis invicta andS. richteri, and their application to the study of a hybrid zone. — Molec. Ecol.3: 531–539.

    Google Scholar 

  • Doyle, J. J., Beachy, R. N., Lewis, W. H., 1984: Evolution of rDNAs inClaytonia polyploid complexes. — InGrant, W. F., (Ed.): Plant biosystematics, pp. 321–341. — London: Academic Press.

    Google Scholar 

  • , 1989: 5s nuclear ribosomal gene variation in theGlycine tomentella polyploid complex. — Syst. Bot.14: 621–624.

    Google Scholar 

  • Feinberg, A. P., Vogelstein, B., 1983: A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. — Ann. Biochem.132: 6–13.

    Google Scholar 

  • Gottlieb, L. D., 1972: Levels of confidence in the analysis of hybridisation in plants. — Ann. Missouri Bot. Gard.59: 435–446.

    Google Scholar 

  • Grant, W. F., (Ed.) 1984: Plant biosystematics. — Toronto, London: Academic Press.

    Google Scholar 

  • Hadrys, H., Balick, M., Schierwater, B., 1992: Applications of random amplified polymorphic DNA (RAPD) in molecular ecology. — Molec. Ecol.1: 55–63.

    Google Scholar 

  • Ham, R. C. H. J. Van, 't Hart, H., Mes, T. H. M., Sandbrink, J. M., 1994: Molecular evolution of non-coding regions of the chloroplast genome in theCrassulaceae and related species. — Curr. Genet.25: 558–566.

    Google Scholar 

  • Harris, S. A., 1995: Systematics and randomly amplified polymorphic DNA in the genusLeucaena (Leguminosae, Mimosoideae). — Pl. Syst. Evol.197: 195–208.

    Google Scholar 

  • , 1993: A molecular study of genetic resources in the genusLeucaena Benth. Final Report. — London: Forestry Research Programme, Overseas Development Administration.

    Google Scholar 

  • , 1994: Genetic variation inLeucaena leucocephala (Lam.)de Wit. (Leguminosae: Mimosoideae). — Silvae Genet.43: 159–167.

    Google Scholar 

  • Hawkins, J. A., 1996: Systematics ofParkinsonia L. andCercidium Tul. (Leguminosae: Caesalpinioideae). — Ph.D. Thesis, University of Oxford, UK.

    Google Scholar 

  • -White Olascoaga, L., Hughes, C. E., Contreras Jiménez, J.-L. R., Mercado Rvaro, P., 1998: Investigation and documentation of hybridization betweenParkinsonia aculeata andCercidium praecox (Leguminosae: Caesalpinioideae). — Pl. Syst. Evol. (in press).

  • Heun, M., Helentjaris, T., 1993: Inheritance of RAPDs in F1 hybrids of corn. — Theor. Appl. Genet.85: 961–968.

    Google Scholar 

  • Hillis, D. M., Moritz, C., Mable, B. K., 1996: Molecular systematics. — Sunderland, Massachusetts: Sinauer Associates.

    Google Scholar 

  • Hughes, C. E., (1998): Monograph ofLeucaena Benth. — Syst. Bot. Monogr.55 (in press).

  • , 1994: The characterisation and identification of a naturally occurring hybrid in the genusLeucaena (Leguminosae: Mimosoideae). — Pl. Syst. Evol.192: 177–197.

    Google Scholar 

  • Levin, D. A., Fransisco-Ortega, J., Jansen, R. K., 1996: Hybridisation and the extinction of rare plant species. — Conservation Biol.10: 10–16.

    Google Scholar 

  • Lorenz, M., Weihe, A., Börner, T., 1994: DNA fragments of organellar origin in random amplified polymorphic DNA (RAPD) patterns of sugar beet (Beta vulgaris L.). — Theor. Appl. Genet.88: 775–779.

    Google Scholar 

  • Mandany, M. H., 1991: Living fences: Somali farmers adopt an agroforetry technology. — Agroforestry Today3: 4–7.

    Google Scholar 

  • Marsolais, J. V., Pringle, J. S., White, B. N., 1993: Assessment of random polymorphic DNA (RAPD) as genetic markers for determining the origin of interspecific lilac hybrids. — Taxon42: 531–537.

    Google Scholar 

  • McDade, L. A., 1995: Hybridisation and phylogenetics. — InHoch, P. C., Stephenson, A. G., (Eds): Experimental and molecular approaches to plant biosystematics, pp. 305–331. — St. Louis: Missouri Botanical Garden.

    Google Scholar 

  • N'Goran, J. A. K., Laurent, V., Rissterucci, A. M., Lanaud, C., 1994: Comparative genetic diversity studies ofTheobroma cacao L. using RFLP and RAPD markers. — Heredity73: 589–597.

    Google Scholar 

  • Perez de la Rosa, J., Harris, S. A., Farjon, A., 1995: Noncoding chloroplast DNA variation in Mexican pines. — Theor. Appl. Genet.91: 1101–1106.

    Google Scholar 

  • Pound, B., Martinez-Cairo, L., 1983:Leucaena: its cultivation and use. — London: Overseas Development Administration.

    Google Scholar 

  • Rieseberg, L. H., 1995: The role of hybridisation in evolution: old wine in new skins. — Amer. J. Bot.82: 944–953.

    Google Scholar 

  • , 1988: A molecular re-examination of introgression betweenHelianthus annuus andH. bolanderi (Compositae). — Evolution42: 227–238.

    Google Scholar 

  • , 1989: Hybridisation in the island endemic,Catalina mahogany. — Conservation Biol.3: 52–58.

    Google Scholar 

  • , 1992: Molecular evidence and plant introgression. — InSoltis, P. S., Soltis, D. E., Doyle, J. J., (Eds): Molecular systematics of plants, pp. 151–176. — London: Chapman & Hall.

    Google Scholar 

  • , 1993: What can molecular and morphological markers tell us about plant hybridisation? — C. R. C. Crit. Rev. Pl. Sci.12: 213–241.

    Google Scholar 

  • , 1995: Character expression, phylogenetic reconstruction and the detection of reticulate evolution. — InHoch, P. C., Stephenson, A. G., (Eds): Experimental and molecular approaches to plant biosystematics. — St. Louis: Missouri Botanical Garden.

    Google Scholar 

  • Rosen, D. E., 1979: Fishes from the upland and intermontane basins of Guatemala: revisionary studies and comparative biogeography. — Bull. Amer. Mus. Nat. Hist.162: 267–376.

    Google Scholar 

  • Smith, J. J., Scott-Craig, J. S., Leadbetter, J. R., Bush, G. L., Roberts, D. L., Fulbright, D. W., 1994: Characterization of random amplified polymorphic DNA (RAPD) products fromXanthomonas campestris and some comments on the use of RAPD products in phylogenetic analysis. — Molec. Phylogenet. Evol.3: 135–145.

    Google Scholar 

  • Soltis, D. E., Soltis, P. S., 1991: Allopolyploid speciation inTragopogon: insights from chloroplast DNA. — Amer. J. Bot.76: 1119–1124.

    Google Scholar 

  • Stace, C. A., 1975: Hybridisation and the flora of the British Isles. — London: Academic Press.

    Google Scholar 

  • , 1989: Plant taxonomy and biosystematics. — London: Edward Arnold.

    Google Scholar 

  • Stewart, J. L., Dunsdon, A. J., Hellin, J. J., Hughes, C. E., 1992: Wood biomass estimation of Central American dry zone trees. — Tropical Forestry Papers 26. — Oxford: Oxford Forestry Institute.

    Google Scholar 

  • Taberlet, P., Gielly, L., Pautou, G., Bouvet, J., 1991: Universal primers for amplification of three non-coding regions of chloroplast DNA. — Pl. Molec. Biol.17: 1105–1109.

    Google Scholar 

  • Thormann, C. E., Ferreira, M. E., Camargo, L. E. A., Tivang, J. G., Osborn, T. C., 1994: Comparison of RFLP and RAPD markers to estimating genetic relationships within and among cruciferous species. — Theor. Appl. Genet.88: 973–980.

    Google Scholar 

  • Wachira, F. N., Waugh, R., Hackett, C. A., Powell, W., 1995: Detection of genetic diversity in tea (Camellia sinesis) using RAPD markers. — Genome38: 201–210.

    Google Scholar 

  • Wagner, W. H., 1969: The role and taxonomic treatment of hybrids. — Bioscience19: 785–789.

    Google Scholar 

  • Wang, W. Y., Pai, R. C., Lai, C. C., Lin, T. P., 1994: Molecular evidence for the hybrid origin ofPaulownia taiwaniana based on RAPD markes and RFLP of chloroplast DNA. — Theor. Appl. Genet.89: 271–275.

    Google Scholar 

  • Wells, H., 1980: Adistance coefficient as a hybridisation index: an example usingMimulus longiflorus andM. flemingii (Scrophulariaceae) from Santa Cruz Island, California. — Taxon29: 52–65.

    Google Scholar 

  • Weir, B. S., 1990: Genetic data analysis: methods for discrete population genetic data. — Sunderland, Massachusetts: Sinauer.

    Google Scholar 

  • Williams, J. G. K., Hanafey, M. K., Rafalski, J. A., Tingey, S. V., 1993: Genetic analysis using random amplified polymorphic DNA markers. — Meth. Enzymol.218: 704–740.

    Google Scholar 

  • Wilson, P., 1992: On inferring hybridity form morphological intermediacy. — Taxon41: 11–23.

    Google Scholar 

  • Woods, W., 1992: Phytophagous insects collected fromParkinsonia aculeata (Leguminosae: Caesalpiniaceae) in the Sonoran Desert region of the southwestern United States and Mexico. — Entomophaga37: 465–474.

    Google Scholar 

  • Zande, L. van de Bijlsma, R., 1995: Limitations of the RAPD technique in phylogeny reconstruction inDrosophila. — J. Evol. Biol.8: 645–656.

    Google Scholar 

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  1. Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB, Oxford, England, UK

    J. A. Hawkins & S. A. Harris

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  1. J. A. Hawkins
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Hawkins, J.A., Harris, S.A. RAPD characterisation of two neotropical hybrid legumes. Pl Syst Evol 213, 43–55 (1998). https://doi.org/10.1007/BF00988907

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