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Fate of iridoid glycosides in different life stages of the Buckeye,Junonia coenia (Lepidoptera: Nymphalidae)

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Abstract

The buckeye butterfly,Junonia coenia (Lepidoptera: Nymphalidae), specializes on plants that contain iridoid glycosides. To determine the fate of these compounds in larvae, pupae, and adults of this species, we reared larvae on artificial diets with and without iridoid glycosides, and on leaves of a host plant,Plantago lanceolata (Plantaginaceae). Quantification by gas chromatography showed that newly molted third-, fourth-, and fifth-instar larvae reared on leaves ofP. lanceolata contained means of 5.13, 2.88, and 6.83% dry weight iridoid glycoside. In contrast, the mean iridoid glycoside concentration of actively feeding fifth-instar larvae was 0.28% dry weight, that of pupae was 0.19% dry weight iridoids, and adults contained no detectable iridoids. Feeding experiments suggested that this reduction in actively feeding larvae was due to the metabolism of iridoid glycosides.P. lanceolata leaves in these experiments contained a mean of 1.00% dry weight iridoid glycoside, with a 2:1 ratio of aucubin to catalpol. Calculation of iridoid consumption and utilization indices showed that larvae fed artificial diets consumed, digested, and sequestered aucubin and catalpol in similar ways. When these indices were calculated for larvae fed leaves ofP. lanceolata, catalpol was sequestered twice as efficiently as aucubin.

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Author notes

  1. Sharon K. Collinge

    Present address: Graduate School of Design, Harvard University, 48 Quincy Street, 02138, Cambridge, Massachusetts

Authors and Affiliations

  1. University of Colorado Museum and Department of E.P.O. Biology, University of Colorado, Campus Box 334, 80309, Boulder, Colorado

    M. Deane Bowers

  2. Museum of Comparative Zoology Harvard University, 02138, Cambridge, Massachusetts

    Sharon K. Collinge

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  1. M. Deane Bowers
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Bowers, M.D., Collinge, S.K. Fate of iridoid glycosides in different life stages of the Buckeye,Junonia coenia (Lepidoptera: Nymphalidae). J Chem Ecol 18, 817–831 (1992). https://doi.org/10.1007/BF00988322

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  • DOI: https://doi.org/10.1007/BF00988322

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