Skip to main content
Log in

Application of Giemsa banding to orchid karyotype analysis

  • Short Communication
  • Published:
Plant Systematics and Evolution Aims and scope Submit manuscript

Abstract

A method for obtaining orchid chromosome squash preparations from ovular tissues and a Giemsa C-band technique are described. Jointly applied, they result in well-defined chromosome banding patterns. Preliminary tests with two species of the genusCephalanthera show that Giemsa banding is also well suited for orchids. Besides aiding in chromosome identification and karyotype analysis, it should prove valuable in studies of chromosomal variation and karyotype evolution of this large family.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Gill, B. S., Kimber, G., 1974: The Giemsa C-banded karyotype of rye. — Proc. Nat. Acad. Sci. USA71, 1247–1249.

    PubMed  Google Scholar 

  • Greilhuber, J., Speta, F., 1976: C-banded karyotypes in theScilla hohenackeri group,S. persica andPuschkinia (Liliaceae). — Pl. Syst. Evol.126, 149–188.

    Google Scholar 

  • Holmquist, G., 1979: The mechanism of C-banding: depurination and β-elimination. — Chromosoma72, 203–224.

    PubMed  Google Scholar 

  • Jones, K., 1974: Cytology and the study of orchids. InWithner, C. L. (Ed.): The orchids: scientific studies, 383–392. — New York-London: John Wiley & Sons.

    Google Scholar 

  • —, 1968: The chromosomes of orchids. III. Catasetinae. — Kew Bull.22, 421–427.

    Google Scholar 

  • Marks, G. E., 1975: The Giemsa-staining centromeres ofNigella damascena. — J. Cell Sci.18, 19–25.

    PubMed  Google Scholar 

  • —, 1977: The nature of centromeric dots inNigella chromosomes. — Chromosoma62, 369–373.

    Google Scholar 

  • Meili-Frei, E., 1966: Cytogenetik und Cytotaxonomie einheimischer Arten vonEpipactis, Listera, Neottia (Orchidaceae). — Ber. Schweiz. Bot. Ges.75, 219–292.

    Google Scholar 

  • Schweizer, D., 1980: Fluorescent chromosome banding in plants: applications, mechanisms, and implications for chromosome structure. In: Proceedings of the 4th John Innes Symposium, “The Plant Genome”, and 2nd International Haploid Conference, pp. 61–72. — Norwich: John Innes Charity.

    Google Scholar 

  • —, 1976: Giemsa banded karyotypes, systematics, and evolution inAnacyclus (Asteraceae-Anthemideae). — Pl. Syst. Evol.126, 107–148.

    Google Scholar 

  • Stack, S. M., 1974: Differential Giemsa staining of kinetochores and nucleolus organizer heterochromatin in mitotic chromosomes of higher plants. — Chromosoma47, 361–378.

    Google Scholar 

  • Withner, C. L., 1974: The orchids: scientific studies. — New York-London: John Wiley & Sons.

    Google Scholar 

  • Yamasaki, N., 1973: Differentielle Darstellung der Metaphasechromosomen vonCypripedium debile mit Chinacrin- und Giemsafärbung. — Chromosoma41, 403–412.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Schwarzacher, T., Ambros, P. & Schweizer, D. Application of Giemsa banding to orchid karyotype analysis. Pl Syst Evol 134, 293–297 (1980). https://doi.org/10.1007/BF00986805

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00986805

Key words

Navigation