Abstract
Branches were cut from young beeches (Fagus sylvatica L.) at various stages of the annual cycle and [3H]indole-3-acetic acid (0.35 nmol) was applied to the whole surface of the apical section of each branch, just below the apical bud. The labelled pulse (moving auxin) and the following weakly radioactive zone (auxin and metabolites retained by the tissues) were localized by counting: microautoradiographss were made using cross sections from these two regions. During the second fortnight of April, auxin was transported by nearly all the cells of the young primary shoot, but the label was more concentrated in the vascular bundles. Auxin transport became the more localized: the cortical parenchyma appeared to lose its ability to transport the hormone (end of April), followed in turn by the pith parenchyma (May). Polar auxin movement at that time was limited to the outer part of the bundle (cambial zone and phloem) and to the inner part (protoxylem parenchyma). Later protoxylem parenchyma ceased to carry auxin. During the whole period of cambial activity, auxin was transported and retained mainly by the cambial zone and its recent derivatives. In September, before the onset of dormancy, and in February, at the end of the resting period, the transport pathways and retention sites for auxin were mainly in the phloem, where sieve tubes often completely lacked radiolabel. When cambial reactivation occurred in the one-year shoot, auxin was mainly carried and retained again in the cambial zone and differentiating derivatives.
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Abbreviations
- IAA:
-
indole-3-acetic acid
References
Bonga, J.M., Clark, J. (1963) Chromatographic detection of seasonal changes in the location of indole-3-acetic acid in balsam fir. Can. J. Bot.41, 1133–1134
Bonnemain, J.L. (1971) Transport et distribution des traceurs après application de AIA-2-C14 sur les feuilles deVicia faba. C.R. Acad. Sci. Ser. D.273, 1699–1702
Bonnemain, J.L. (1972) La plante supérieure, siège d'une circulation complexe. Physiol Vég.10, 697–729
Bonnemain, J.L. (1978) Some aspects of the physiology of collateral and bicollateral bundles. In: Inaugural meeting, F. E. S. P. P., Edinburgh, Symp.: Transport in plants, pp. 11–12. Edinburgh University, Edinburgh
Bonnemain, J.L. (1980) Microautoradiography as a tool for the recognition of phloem transport. Ber. Dtsch. Bot. Ges.93, 99–107
Bourbouloux, A., Bonnemain, J.L. (1973) Transport de l'auxine-14C en provenance de jeunes gousses deVicia faba L. Planta115, 161–172
Bourbouloux, A., Bonnemain, J.L. (1974) Transport, distribution et métabolisme de l'auxine dans la racine deVicia faba L., après application de [C14]AIA ou de[H3]AIA sur le bourgeon. Planta119, 169–182
Bourbouloux, A., Bonnemain, J.L. (1979) The different components of the movement and the areas of retention of labelled molecules after application of [3H]indolyl-acetic acid to the apical bud ofVicia faba. Physiol. Plant.47, 260–268
Bourbouloux, A., Bounemain, J.L., Vasseur, J. (1983) Caractéristiques du transport dans la tige de l'auxine-14C en provenance des jeunes feuilles deVicia faba L. C.R. Acad. Sci. Ser. D276, 501–504
Catesson, A.M. (1981) Le cycle saisonnier des cellules cambiales chez quelques feuillus. Bull. Soc. Bot. Fr. Actual. Bot.128, 43–51
Eschrich, W., Fritz, E. (1972) Microautoradiography of water soluble inorganic ions. In: Microautoradiography and electron probe analysis, pp. 99–112, Lüttge, U., ed. Springer, Berlin Heidelberg New York
Everat-Bourbouloux, A. (1982) Transport et métabolisme de l'auxine et de l'acide abscissique chezVicia faba L. Thèse, Poitiers
Goldsmith, M.H.M. (1977) The polar transport of auxin. Annu. Rev. Plant. Physiol.28, 439–478
Jacobs, M., Gilbert, S.F. (1983) Basal localization of the presumptive auxin transport carrier in pea stem cells. Science220, 1297–1300
Lachaud, S., Bonnemain, J.L. (1982) Xylogenèse chez les Dicotylédones arborescentes. III. Transport de l'auxine et activité cambiale dans les jeunes tiges de Hêtre. Can. J. Bot.60, 869–876
Miginiac, E., Sossountzov, L., Dugue, N. (1978) Modalités du transport et du métabolisme de l'AIA-14C ou de l'AIA-3H en relation avec la morphogenèse des bourgeons axillaires chez leScrophularia argutd. Physiol. Plant.44, 335–344
Morris, D.A., Kadir, G.O. (1972) Pathways of auxin transport in the intact pea seedling (Pisum sativum L.) Planta107, 171–182
Morris, D.A., Thomas, A.G. (1978) A microautoradiographic study of auxin transport in the stem of intact pea seedlings (Pisum sativum L.). J. Exp. Bot.29, 147–157
Nix, L.E., Wodzicki, T.J. (1974) The radial distribution and metabolism of IAA-14C inPinus echinata stems in relation to wood formation. Can. J. Bot.52, 1349–1355
Sheldrake, A.R. (1973) Auxin transport in secondary tissues. J. Exp. Bot.24, 87–96
Tvision, Y., Zamski, E. (1977) Translocation in plants possessing supernumerary phloem. II. The included phloem ofBougainvillea glabra Choisy and the bicollateral bundles of cucumber (Cucumis sativus L.). J. Exp. Bot.28, 127–131
Wangermann, E. (1974) The pathway of transport of applied indolyl-acetic acid through internode segments. New Phytol.73, 623–636
Wangermann, E., Withers, L.A. (1978) Auxin transport characteristics and cellular ultrastructure of different types of parenchyma. New Phytol.81, 1–17
Zamski, E., Tvision, Y. (1977) Translocation in plants possessing supernumerary phloem. I.14C-assimilates and auxin in the internal phloem of tobacco (Nicotiana tabacum L.). J. Exp. Bot.28, 117–126
Zamski, E., Wareing, P.F. (1974) Vertical and radial movement of auxin in young sycamore plants. New Phytol.73, 61–69
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Lachaud, S., Bonnemain, J.L. Seasonal variations in the polar-transport pathways and retention sites of [3H]indole-3-acetic acid in young branches ofFagus sylvatica L.. Planta 161, 207–215 (1984). https://doi.org/10.1007/BF00982914
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DOI: https://doi.org/10.1007/BF00982914