Abstract
“Nitrogen surplus” models for nicotine production induced by leaf damage predict that the observed increase in root nicotine synthesis after leaf damage results from “overflow” metabolism; reduced nitrogen existing in excess of growth requirements is shunted into nicotine biosynthesis. To test the nitrogen surplus model for induced nicotine production, we measured the concentrations of the majorN-containing metabolites exported from the roots and the nitrate reductase activity (NRA) of roots and shoots in damaged and undamagedNicotiana sylvestris plants. Leaf damage: (1) had no significant effect on root or shoot NRA, (2) increased nicotine export and decreased amino-acid and amide export from the roots of NO3-fertilized plants, and (3) had no significant effect on the export of anyN-containing metabolite from the roots of NH4-fertilized plants. These results are not consistent with the nitrogen surplus model and indicate that leaf damage has a direct influence on root alkaloid metabolism.
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Baldwin, I.T., Oesch, R.C., Merhige, P.M. et al. Damage-induced root nitrogen metabolism inNicotiana sylvestris: Testing C/N predictions for alkaloid production. J Chem Ecol 19, 3029–3043 (1993). https://doi.org/10.1007/BF00980600
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DOI: https://doi.org/10.1007/BF00980600