Abstract
The permeation of labeled Cl− ions across single plasma membranes from Deiter's neurons has been studied in the presence of various concentrations of phosphatidylserine (PS) on their extracellular side. PS reduces significantly basal Cl− permeation only at 10−5 M on the membrane exterior. No effect was found at other concentrations. GABA activable36Cl− permeation is heavily reduced and almost abolished at 10−11–10−5 M phosphatidylserine. This exogenous phosphatidylserine effect is difficult to interpret in relation to the function of the endogenous phospholipid. However, it may be involved in the epileptogenic effect in vivo of exogenous phosphatidylserine administration to rats.
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References
Giambalvo, C. T., and Rosenberg, P. 1976. The effect of phospholipases and proteases on the binding of γ-amino-butyric acid to junctional complexes of rat cerebellum. Biochim. Biophys. Acta. 436:741–756.
Lloyd, K. G., and Davidson, L. 1979.3H-GABA binding in brains from Hintington's chorea patients: altered regulation by phospholipid. Science. 205:1147–1149.
Toffano, G., Aldinio, C., Balzano, M., Leon, A., and Savoini, G. 1987. Regulation of GABA receptor binding to synaptic plasma membrane of rat cerebral cortex: the role of endogenous phospholipids, Brain Res. 222:95–102.
Ueno, E., and Kuriyama, K. 1981. Phospholipids and benzodiazepine recognition sites of brain synaptic membranes, Neuropharmacology. 20:1169–1176.
Fujimoto, M., and Okabayashi, T. 1983. Influence of phospholipase treatments on ligand bindings to a benzodiazepine receptor-GABA receptor-chloride ionophore complex. Life Sciences, 32:2393–2400.
Hydén, H., Lange, P. W., and Larsson, S. 1980. S-100 glia regulation of GABA transport across the nerve cell membrane, J. Neurol. Sci. 45:303–316.
Rapallino, M. V., Cupello, A., and Hydén, H. 1988. Direct evidence for the presence of GABAA receptors on the cytoplasmic side of the Deiters' neurone membrane, Brain Res., 462:350–353.
Hydén, H., Cupello, A., and Palm, A. 1986. γ-amino-butyric acid stimulates chloride permeability across microdissected Deiters' neuronal membrane, Brain Res. 379:167–170.
Schofield, P. R., Darlison, M. G., Fujita, N., Burt, D. R., Stephenson, F. A., Rodriguez, H., Rhee, L. M., Ramachandran, J., Reale, V., Glencorse, T. A., Seeburg, P. H. and Barnard, E. A. 1987. Sequence and functional expression of the GABAA receptor shows a ligand-gated receptor super-family, Nature. 328:221–227.
Martin, T. W., and Lagunoff, D. 1978. Interaction of phosphatidylserine with mast cells, Proc. Nat. Acad. Sci. USA. 75:4997–5000.
Loeb, C. W., Besio, G., Mainardi, P., Scotto, P. A., Benassi, E., and Bo, G. P. 1986. Liposome-entrapped γ-aminobutyric acid inhibits isoniazid-induced epileptogenic activity in rats. Epilepsia. 27:98–102.
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Rapallino, M.V., Cupello, A., Mainardi, P. et al. Effect of phosphatidylserine on the basal and GABA-activated Cl− permeation across single nerve membranes from rabbit Deiters' neurons. Neurochem Res 15, 593–596 (1990). https://doi.org/10.1007/BF00973748
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DOI: https://doi.org/10.1007/BF00973748