Abstract
Several aspects of energy metabolism (glucose utilization, lactate production,14CO2 production from labeled glucose, glutamate or pyruvate, oxygen consumption and contents of ATP and phosphocreatine) were measured in cerebellar granule cells (glutamatergic) in primary cultures and compared with corresponding data for cerebral cortical neurons (mainly GABA-ergic) and astrocytes. Cerebellar granule cells and astrocytes were metabolically more active than cerebral cortical neurons. Glutamate which is utilized as a major metabolic fuel as astrocytes and, to a lesser extent, in cerebral cortical neurons, was virtually not oxidized in cerebellar granule cells.
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Hydén, H., and Pigon, A. 1960. A cytophysiological study of the functional relationship between oligodendroglial cells and nerve cells of Deiters' nucleus. J. Neurochem. 6:57–72.
Hertz, L., Juurlink, B. H. J., Szuchet, S., and Walz, W. 1985. Cell and tissue culture, Pages 117–167,in Boulton, A. A. and Baker, G. B. (eds.) Neuromethods, Vol. 2. Humana Press, Clifton, New Jersey.
Hertz, L. 1978. Energy metabolism of glial cells. Pages 121–132,in Schoffeniels, E., Franck, G., Hertz, L. and Tower, D. B. (eds.). Dynamic Properties of Glia Cells, Pergamon Press, Oxford.
Schousboe, A., Drejer, J., and Hertz, L. 1985. Comparison of the metabolites of glucose and glutamate in cultured cerebellar granule cells. J. Neurochem. 44:S168B.
Schousboe, A., Drejer, J., Hansen, G. H. and Meier, E. 1985. Cultured neurons as model systems for biochemical and pharmacological studies on receptors for neurotransmitter amino acids. Dev. Neurosci. 7:252–262.
Messer, A. 1977. The maintenance and identification of mouse cerebellar granule cells in monolayer culture. Brain Res. 130:1–12.
Hertz, L., and Schousboe, A. 1987. Primary cultures of GABAergic and glutamatergic neurons as model systems to study neurotransmitter functions. I. Differentiated cells. Pages 19–31,in Model Systems of Development and Aging of the Nervous System. Vernadakis, A., Privat, A., Lauder, J. M., Timiras, P. S. and Giacobini, E. (eds.). Martinus Nijhoff Publishers, Mass.
Schousboe, A., and Hertz, L., Primary cultures of GABAergic and glutamatergic neurons as model systems to study neurotransmitter functions. II. Developmental Aspects. Pages 33–35,in Model Systems of Development and Aging of the Nervous System. Vernadakis, A., Privat, A., Lauder, J. M., Timiras, P. S. and Giacobini, E. (eds.). Martinus Nijhoff Publishers, Mass.
Yavin, Z., and Yavin, E. 1980. Survival and maturation of cerebral neurons on poly(l-lysine) surfaces in the absence of serum. Dev. Biol. 75:454–459.
Booher, J., and Sensenbrenner, M. 1982. Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures. Neurobiol. 2:97–105.
Hertz, E., and Hertz, L. 1979. Polarographic measurement of oxygen uptake by astrocytes in primary cultures using the tissue culture flask as the respirometer chamber. In Vitro 15:429–436 (1979).
Yu, A. C. H., Schousboe, A., and Hertz, L. 1982. Metabolic fate of [14C]-labelled glutamate in astrocytes. J. Neurochem. 39:954–966.
Sykes, J. E. C., Lopes-Cardozo, M., and Van Den Bergh, S. G. 1986. Substrate utilization for energy production and lipid synthesis in oligodendrocyte enriched cultures prepared from rat brain. Neurochem. Int. 8:67–75.
Lopes-Cardozo, M., Larsson, O. M. and Schousboe, A. 1986. Acetoacetate and glucose as lipid precursors and energy substrates in primary cultures of astrocytes and neurons from mouse cerebral cortex. J. Neurochem. 46:773–778.
Heinz, F., and Weisser, H. 1985. Creatine phosphate. Pages 507–514in Bergmeyer, H. U., Bergmeyer, J. and Grassl, M. Methods of Enzymatic Analysis, 3rd Edition. Vol. 8, VCH Verlagsgesellschaft, Weinheim, Germany.
Fitzpatrick, S. M., Cooper, A. J. L., and Hertz, L. 1988. Effect of ammonia and β-methylene-D.L-aspartate on the oxidation of glucose and pyruvate by neurons and astrocytes in primary cultures. J. Neurochem. submitted.
Matz, H., and Hertz, L. 1988. Adenosine metabolism in neurons and astrocytes in primary cultures. Trans. Amer. Soc. Neurochem. in press.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. L. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:265–275.
Cummins, C. J., Lust, W. D., and Passonneau, J. V. 1983. Regulation of glycogen metabolism in primary and transformed astrocytes in vitro. J. Neurochem. 40:128–136.
Lajtha, A., Maker, H. S., and Clarke, D. D. 1981. Chapter 17, Metabolism and transport of carbohydrates and amino acids. Pages 329–353,in Siegel, G. J., Albers, W., Agranoff, B. W. and Katzman, R., (eds.), Basic Neurochemistry, 3rd Edition. Little, Brown and Company, Boston.
Yu, A. C. H., and Hertz, L. 1983. Metabolic sources of energy in astrocytes. Pages 431–439,in Hertz, L., Kvamme, E., McGeer, E. G. and Schousboe, A. (eds.), Glutamine, Glutamate and GABA in the Central Nervous System. Alan R. Liss, New York.
Edmond, J., Auestad, N., Robbins, R. A., and Bergstrom, J. D. 1985. Ketone body metabolism in the neonate: Development and the effect of diet. Fed. Proc. 44:2359–2364.
Elliott, K. A. C., and Henderson, N. 1948. Metabolism of brain tissue slices and suspensions from various mammals. J. Neurophysiol. 11:471–484.
Hertz, L. 1981. Features of astrocytic function apparently involved in the response of central nervous tissue to ischemia-hypoxia. J. Cerebr. Blood Flow Metab. 1:143–153.
Hertz, E., Shargool, M., and Hertz, L. 1986. Effects of barbiturates on energy metabolism by cultured astrocytes and neurons in the presence of normal and elevated potassium concentrations. Neuropharmacol. 25:533–539.
Jameson, N., Olson, J., Nguyen, H., and Holtzman, D. 1984. Respiration in primary cultured cerebellar granule neurons and cerebral cortical neurons. J. Neurochem. 42:470–474.
Olson, J. E., and Holtzman, D. 1981. Factors influencing the growth and respiration of rat cerebral astrocytes in primary culture. Neurochem. Res. 6:1337–1343.
Roth-Schechter, B., Sensenbrenner, M., and Mandel, P. 1976. Effet du pentobarbital sur la morphologie et la respiration des cellules gliales en culture primaire, C. R. Acad. Sci. Paris Serie D 283:1333–1335.
Peterson, A., Odland, L., Sensenbrenner, M., and Walum, E. 1986. Oxidative metabolism in cultured astroglial cells from rat brain. Int. J. Neurosci. 4:35–39.
Yu, A. C. H., Schousboe, A., and Hertz, L. 1984. Influence of pathological concentrations of ammonia on metaboic fate of14C-labeled glutamate in astrocytes in primary cultures. J. Neurochem. 42:594–597.
Murthy, Ch. R. K., and Hertz, L. 1987. Pyruvate decarboxylation in astrocytes and in neurons in primary cultures in the presence and absence of ammonia. Neurochem. Res., in press.
Schousboe, A., Booher, J., and Hertz, L. 1970. Content of ATP in cultivated neurons in astrocytes exposed to balanced and potassium-rich media. J. Neurochem. 17:1501–1504.
Tildon, J. T., and Roeder, L. M. 1984. Glutamine oxidation by dissociated cells and homogenates of rat brain: Kinetics and inhibitor studies. J. Neurochem. 42:1069–1076.
Aoki, C., Milner, T. A., Berger, S. B., Sheu, K.-F. F., Blass, J. P., and Pickel, V. M. 1987. Glial glutamate dehydrogenase: ultrastructural localization and regional distribution in relation to the mitochondrial enzyme, cytochrome oxidase. J. Neurosci. Res. 18:305–318.
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Special Issue dedicated to Prof. Holger Hydén.
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Hertz, L., Drejer, J. & Schousboe, A. Energy metabolism in glutamatergic neurons, GABAergic neurons and astrocytes in primary cultures. Neurochem Res 13, 605–610 (1988). https://doi.org/10.1007/BF00973275
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DOI: https://doi.org/10.1007/BF00973275