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Glutaminase in neurons and astrocytes cultured from mouse brain: Kinetic properties and effects of phosphate, glutamate, and ammonia

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Abstract

Phosphate activated glutaminase comprises two kinetically distinguishable enzyme forms in cultures of cerebellar granule cells, of cortical neurons and of astrocytes. Specific activity of glutaminase is higher in cultured neurons compared with astrocytes. Glutaminase is activated by phosphate in all cell types investigated, however, glutaminase in astrocytes reguires a much higher concentration of phosphate for half maximal activation. One of the products, glutamate, inhibits the enzyme strongly, whereas the other product ammonia has only a slight inhibitory action on the enzyme.

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Authors and Affiliations

  1. Neurochemical Laboratory, University of Oslo, Blindern, Postbox 1115, 0317, Oslo 3, Norway

    S. Hogstad, G. Svenneby, I. Aa. Torgner & E. Kvamme

  2. Department of Pharmacology, University of Saskatchewan, S7N 0W0, Saskatoon, Saskatchewan, Canada

    L. Hertz

  3. Department of Biochemistry A, Panum Institute, University of Copenhagen, DK-2200, Copenhagen N, Denmark

    A. Schousboe

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  1. S. Hogstad
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  2. G. Svenneby
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  3. I. Aa. Torgner
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  4. E. Kvamme
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  5. L. Hertz
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  6. A. Schousboe
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Hogstad, S., Svenneby, G., Torgner, I.A. et al. Glutaminase in neurons and astrocytes cultured from mouse brain: Kinetic properties and effects of phosphate, glutamate, and ammonia. Neurochem Res 13, 383–388 (1988). https://doi.org/10.1007/BF00972489

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