Abstract
The effect of lithium on the sodium-dependent high-affinity system for tryptophan uptake was examined in plasma membrane vesicles derived from rat brain. We demonstrated that Na+ could be replaced by lithium in the external medium and the presence of lithium produced an increase in theV max of the tryptophan transport whereas it had no significant effect on theK m for the substrate. Plasma membrane vesicles derived from synaptosomes obtained from long-term lithium-treated rats are able to accumulate tryptophan to a greater extent than normal rats and maintain a more negative membrane potential than controls. Our data support the idea that the stimulation by lithium of the high-affinity uptake system for tryptophan by maintaining adequate membrane potentials across the membrane, could lead to the stabilization of serotonin production, as has been demonstrated in long termlithium treatment.
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References
Sheard, M. H. 1980. The biological effects of lithium. TINS 3:85–86.
Ehrlich, B. E., and Diamond, J. M. 1980. Lithium, membranes and manic-depressive illness. J. Membrane Biol. 52:187–200.
Sherman, W. R., Munsell, L. Y., Beverly, G. G., and Honchar, M. P. 1985. Effects of systematically administered lithium on phosphoinositide metabolism in rat brain, kidney and testis. J. Neurochem. 44:798–807.
Schou, M., and Thomsen, K. 1975. Lithium prophylaxis of recurrent endogenous affective disorders. Pages 63–84, in Johnson, F. N. (ed.), Lithium research and therapy, Academic Press, London.
Perez-Creuet, J., Tagliamonte, P., and Gessa, G. L. 1971. Stimulation of serotonin synthesis by lithium. J. Pharmacol. Exp. Ther. 178:325–330.
Mandell, A. J., and Knapp, S. 1977. Regulation in serotonin biosynthesis in brain: role of the high affinity uptake of tryptophan into serotonergic neurons. Fed. Proc. Fed. Am. Soc. Exp. Biol. 36:2142–2148.
Wajda, I. J., Banay-Schwartz, M., Manigault, I., and Lajtha, A. 1978. Effects of lithium and sodium ions on opiate- and dopamine-receptor binding. Neurochem. Res. 6:321–331.
Pert, A., Rosenblatt, J. E., Sivit, C., Pert, C. B., and Bunney, W. E. 1978. Long-term treatment with lithium prevent the development of dopamine receptor supersensitivity. Science. 201:171–173.
Laakso, M.-L., and Oja, S. S. 1979. Transport of tryptophan and tyrosine in rat brain slices in the presence of lithium. Neurochem. Res. 4:411–423.
Treiser, S., and Kellar, K. J. 1980. Lithium: effects on serotonin receptors in rat brain. Eur. J. Pharmacol. 58:85–86.
Knapp, S., and Mandell, A. J. 1973. Short- and long-term lithium administration: effects on the brain's serotonergic biosynthetic systems. Science 180:645–647.
Mandell, A. J., and Knapp, S. 1975. Neurobiological mechanisms in lithium prophylaxis of manic-depressive disease: an hypothesis. Pages 9–16 in Almgrem, O., Carlsson, A. and Engel, J. (eds.), Chemical tools in catecholamine research II, North-Holland, Amsterdam.
Sangee, and Franz, D. V. 1978. Lithium-induced enhancement of 5-HT transmission at a central synapse. Commun. Psychopharmacol. 2:191–198.
Friedman, E., Dallob, A., and Levine, G. 1979. The effects of long-term lithium treatment on reserpine-induced supersensitivity in dopaminergic and serotonergic transmission. Life Sci. 25:1263–1266.
Meek, J. L., and Lofstrand, S. 1976. Tryptophan hydroxylase in discrete brain nuclei: comparison of activity in vitro and in vivo. Eur. J. Pharmacol. 37:377–380.
Mc Kean, C. M., Boggs, D. E., and Peterson, N. A. 1968. The influence of high phenylalanine and tyrosine on the concentration of essential amino acids in brain. J. Neurochem. 15:235–241.
Tyfield, L. A., and Holton, J. B. 1976. The effect of high concentration of histidine on the level of other amino acids in plasma and in brain of the mature rat. J. Neurochem. 26:101–105.
Grahame-Smith, D. G., and Parfitt, A. G. 1970. Tryptophan transport across the synaptosomal membrane. J. Neurochem. 17:1339–1353.
Lajtha, A. 1974. Amino acid transport in the brain in vivo and in vitro. Pages 25–41 in Wolstenholme, G. E. W. and Fitzsmons, D. W. (eds.), Aromatic Amino Acids in the Brain, Elsevier, Amsterdam.
Korpi, E. R. 1980. Tryptophan and phenylalanine transport in rat cerebral cortex slices as influenced by sodium ions. Neurochem. Res. 5:415–431.
Herrero, E., Aragon, M. C., Gimenez, C., and Valdivieso, F. 1983. Tryptophan transport into plasma membrane vesicles derived from rat brain synaptosomes. J. Neurochem. 40:332–337.
Shultz, S. G., and Curran, P. F. 1970. Coupled transport of sodium and organic solutes. Physiol. Rev. 50:637–718.
West, I. C. 1980. Energy coupling in secondary transport. Biochim. Biophys. Acta 604:91–126.
Kanner, B. I. 1983. Bioenergetics of neurotransmitter transport. Biochim. Biophys. Acta 726:239–316.
Kanner, B. I. 1978. Active transport of γ-aminobutyric acid by membrane vesicles isolated from rat brain. Biochemistry 17:1207–1211.
Aragon, M. C., Gimenez, C., Mayor, F. Jr., Marvizon, J. G., and Valdivieso, F. 1981. Tyrosine transport into plasma membrane vesicles derived from rat brain synaptosomes. Biochim. Biophys. Acta 646:465–470.
Zafra, F., Aragon, M. C., Valdivieso, F., and Gimenez, C. 1984. β-Alanine transport into plasma membrane vesicles derived from rat brain synaptosomes. Neurochem. Res. 9:695–707.
Herrero, E., Aragon, M. C., Diez-Guerra, J., Valdivieso, F., and Gimenez, C. 1985. Ontogenic studies on tryptophantransport into plasma membrane vesicles derived from rat brain synaptosomes: effect of thyroid hormones. Neurochem. Res. 10:579–589.
Scott, I. D., and Nicholls, D. G. 1980. Energy transduction in intact synaptosomes. Biochem. J. 186:21–33.
Lichtshtein, D., Kaback, H. R., and Blume, A. J. 1979. Use of lipophilic cation for determination of membrane potential in neuroblastoma-glioma hybrid cell suspensions. Proc. Natl. Acad. Sci. USA 76:650–654.
Resch, K., Imm, I., Ferber, E., Wallaach, D. F. H., and Fisher, H. 1971. Quantitative determination of soluble and membrane proteins through their native fluorescence. Naturwissennschaften. 58:220.
Korpi, E. R. 1983. Kinetics of tryptophan influx into brain slices depleted of sodium and loaded withl-histidine. J. Neurochem. 40:428–433.
Korpi, E. R., and Oja, S. S. 1983. Mechanism ofl-histidine-induced stimulation of amino acid transport in slices of rat cerebral cortex. Neurochem. Int. 5:793–801.
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Aragon, M.C., Herrero, E. & Gimenez, C. Effects of systematically administered lithium on tryptophan transport and exchange in plasma-membrane vesicles isolated from rat brain. Neurochem Res 12, 439–444 (1987). https://doi.org/10.1007/BF00972295
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DOI: https://doi.org/10.1007/BF00972295