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Osmotic regulation ofmyo-inositol uptake in primary astrocyte cultures

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Abstract

Uptake ofmyo-inositol by astrocytes in hypertonic medium (440 mosm/kg H2O) was increased near 3-fold after incubation for 24 hours, which continued for 72 hours, as compared with the uptake by cells cultured in isotonic medium (38 nmoles/mg protein).myo-Inositol uptake by astrocytes cultured in hypotonic medium (180 mosm/kg H2O) for periods up to 72 hours was reduced by 74% to 8 to 10 nmoles/mg protein. Astrocytes incubated in either hypotonic or hypertonic medium for 24 hours and then placed in isotonic medium reversed the initial down- or up-regulation of uptake. Activation of chronic RVD and RVI correlates with regulation ofmyo-inositol uptake. A 30 to 40 mosm/kg H2O deviation from physiological osmolality can influencemyo-inositol homeostasis. The intracellular content ofmyo-inositol in astrocytes in isotonic medium was 25.6 ± 1.3 μg/mg protein (28 mM). This level ofmyo-inositol is sufficient for this compound to function as an osmoregulator in primary astrocytes and it is likely to contribute to the maintenance of brain volume.

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Authors and Affiliations

  1. Research Service (151), Veterans Affairs Medical Center, 1201 N.W. 16th Street, 33125, Miami, Florida

    Russell E. Isaacks Ph. D., Alexander S. Bender, Chang Y. Kim, Nicole M. Prieto & Michael D. Norenberg

  2. Department of Medicine, and Laboratory of Neuropathology, University of Miami School of Medicine, 33125, Miami, Florida

    Russell E. Isaacks Ph. D., Chang Y. Kim & Nicole M. Prieto

  3. Department of Pathology, University of Miami School of Medicine, 33125, Miami, Florida

    Alexander S. Bender & Michael D. Norenberg

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Isaacks, R.E., Bender, A.S., Kim, C.Y. et al. Osmotic regulation ofmyo-inositol uptake in primary astrocyte cultures. Neurochem Res 19, 331–338 (1994). https://doi.org/10.1007/BF00971582

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