Abstract
The effect of reserpine (2 mg/kg i.p.) on both locomotor activity and the turnover of dopamine metabolite in the rat nucleus accumbens was estimated by using an activity monitor (Animex) and by in vivo brain microdialysis. Three to five hours after reserpine administration locomotor activity was reduced and there was a concomitant increase in the level of the dopamine metabolite, homovamillic acid. These findings suggest that depletion of dopamine from the nucleus accumbens may result in decreased locomotor activity. The data support the notion that dopamine in this tissue contributes to the control of locomotion.
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Elkhawad, A. O., and Woodruff, G. N. 1975. Studies on the behavioural pharmacology of a cyclic analogue of dopamine following its injection into the brain of conscious rats. Br. J. Pharmacol. 54:107–114.
Grabowska, M., and Andén, N. E. 1976. Apomorphine in the rat nucleus accumbens: Effect on the synthesis of 5-hydroxytryptamine and noradrenaline, the motor activity and the body temperature. J. Neur. Transm. 38:1–8.
Wachtel, H., Ahlenius, S., and Anden, N. E. 1979. Effects of locally applied dopamine to the nucleus accumbens on the motor activity of normal rats and following α-methyltyrosine or reserpine. Psychopharmacol. 63:203–206.
Kelly, P. H., Seviour, P. W., and Iversen, S. D. 1975. Amphetamine and apomorphine responses in the rat following 6-OHDA lesion of the nucleus accumbens septi and corpus striatum. Brain Res. 94:507–522.
Ungerstedt, U., Herrera-Marschitz, M., Jungnelius, U., Stahle, L., Tossman, U., and Zetterström, T. 1982. Dopamine synaptic mechanisms reflected in studies combining behavioral recordings and brain dialysis. Adv. Biosci. 37:219–231.
Di Dhiara, G., and Imperato, A. 1985. Ethanol preferentially stimulates dopamine release in the nucleus accumbens of freely moving rats. Eur. J. Pharmacol. 115:131–132.
Imperato, A. and Di Chiara, G. 1986. Preferential stimulation of dopamine release in the nucleus accumbens of freely moving rats by ethanol. J. Pharmacol. Exp. Therap. 239:219–228.
Sharp, T., Zetterstrom, T., Ljungberg, T. and Ungerstedt, U. 1987. A direct comparison of amphetamine-induced behaviours and regional brain dopamine release in the rat using intercerebral dialysis. Brain Res. 401:322–330.
Westerlink, B. H. 1979. Further studies on the sequence of dopamine metabolism in the rat brain. Eur. J. Pharmacol. 56:313–322.
Studler, J. M., Reibaud, M., Tramu, G., Blanc, G., Glowinski, J., and Tassin, J. P. 1984. Pharmacological study on the mixed CCK/DA meso-nucleus accumbens pathway: Evidence for the existence of storage sites containing the two transmitters. Brain Res. 298:91–97.
König, J. F. R. and Klippel, R. A. 1963, The Rat Brain. Williams & Wilkins, Baltimore.
Hutson, P. H., Sarna, B. S., Kantamanei, B. D. and Curzon, G. 1985. Monitoring the effect of tryptophan load on brain indole metabolism in freely moving rats by simultaneous cerebrospinal fluid sampling and brain dialysis. J. Neurochem. 44:1266–1273.
Johnson, R. D. and J. B. Justice, 1983. Model studies for brain dialysis. Brain Res. Bull. 10:567–571.
Sarna, G. S., Hutson, P. H., and Curzun, G. 1984. Effect of α-methylfluorodopa on dopamine metabolites: Importance of conjugation and egress. Eur. J. Pharmacol. 100:343–350.
Hernandez, L., Stanley, B. G. and Hoebel, B. G. 1986. A small, removable microdialysis probe. Life Sci. 39:2629–2639.
Imperato, A., and Di Chiara, G. 1984. Trans-striatal dialysis coupled to reverse phase high performance liquid chromatography with electrochemical detection: A new method for the study of the in vivo release of endogeneous dopamine and metabolites. J. Neuorosi. 4:966–977.
Butcher, S. P., Fairbrother, J. S., and Arbuthnott, G. W. 1988. Amphetamine-induced dopamine release in the rat striatum: An in vivo microdialysis study. J. Neurochem. 50:346–355.
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Sugita, R., Sawa, Y., Nomura, S. et al. Effects of reserpine on dopamine metabolite in the nucleus accumbens and locomotor activity in freely moving rats. Neurochem Res 14, 267–270 (1989). https://doi.org/10.1007/BF00971322
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DOI: https://doi.org/10.1007/BF00971322