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Brain 12-HETE formation in different species, brain regions, and in brain microvessels

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Abstract

We used gas chromatography/mass spectrometry to measure brain 12-HETE (12-Hydroxy-5,8,10,14-eicosatetraenoic acid) formation from endogenous arachidonic acid in different species and different brain regions and in isolated brain microvessels. When blood-free brain slices were incubated for 20 minutes we found that the rabbit and cat brain incubates contained little 12-HETE when compared to rat and mouse brain incubates. Further in vitro studies of various rat brain regions showed a generally even distribution of 12-HETE. When isolated rat or rabbit microvessels were incubated and analyzed, we found 1 and 0.25 μg, respectively, of 12-HETE/mg of microvessel protein. Also, rabbit brain had limited or no capacity to actively metabolize tritiated 12-HETE. In summary, these studies show substantial species variation with respect to brain formation of 12-HETE and indicate that the vasculature is a potentially significant contributor to the 12-HETE found in whole brain tissue.

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Author notes

  1. Gary S. Hambrecht

    Present address: Nova Pharmaceutical Corp., 5210 Eastern Ave., 21224, Baltimore, MD

  2. Sunday A. Adesuyi

    Present address: Department of Mathematics and Science, St. Paul's College, 23868, Lawrenceville, VA

Authors and Affiliations

  1. Department of Pharmacology and Toxicology, Medical College of Virginia, Virginia Commonwealth Univesity, Richmond, Virginia, USA

    Gary S. Hambrecht, Sunday A. Adesuyi, Sallie Holt & Earl F. Ellis

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  1. Gary S. Hambrecht
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  2. Sunday A. Adesuyi
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  3. Sallie Holt
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  4. Earl F. Ellis
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Hambrecht, G.S., Adesuyi, S.A., Holt, S. et al. Brain 12-HETE formation in different species, brain regions, and in brain microvessels. Neurochem Res 12, 1029–1033 (1987). https://doi.org/10.1007/BF00970932

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  • DOI: https://doi.org/10.1007/BF00970932

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