Skip to main content
SpringerLink
Log in

Depolarization of the neuronal membrane caused by Co-transport of taurine and sodium

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

C 1300 neuroblastoma cells were cultured and used to study the effect of sodium dependent taurine transport on the membrane potential. Measuring net accumulation of taurine and the depolarization caused by externally applied taurine, we found both processes become active at an external concentration of taurine of 1 mM or more. Net accumulation had Km of 13 mM and a Vmax of 126 nmol × mg of protein−1×min−1. The taurine induced depolarization of the neuroblastoma cell was parallelled by a 25 per cent decrease in its membrane impedance. The transport of taurine, the depolarization caused by taurine and the effect of taurine on the membrane impedance, all, had a similar dependence on the external sodium concentration. Our results on the depolarizing cotransport between taurine and sodium at the neuronal membrane, may illustrate an additional mechanism for the control of the electrical activity of neuronal cells.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kontro, P. and Oja, S. S. 1983. Mutual interaction in the transport of taurine, hypotaurine and GABA in brain slices. Neurochem. Res. 8:1377–1387.

    Google Scholar 

  2. Lerma, J., Herreras, O., Herranz, A. S., Munoz, D., and Del Rio, R. M. 1984.In vivo effects of nipecotic acid on levels of extra cellular GABA and taurine, and hippocampal exitability. Neuropharmacology. 23:595–598.

    Google Scholar 

  3. Lajtha, A., and Sershen, H. 1975. Inhibition of amino acid uptake by the absence of Na+ in slices of brain. J. Neurochem. 24:667–672.

    Google Scholar 

  4. Schmid, R., Sieghart, W., and Karobath, M. 1975. Taurine uptake in synaptosomal fractions of rat cerebral cortex. J. Neurochem. 25:5–9.

    Google Scholar 

  5. Martin, D.L. 1976. Carrier-mediated transport and removal of GABA from synaptic regions. In: GABA in Nervous System Function (E. Roberts, T.N. Chase and D.B. Tower, eds.), pp. 347–386, Raven Press, New York.

    Google Scholar 

  6. Blaustein, M.P., and King, A.Ch. 1976. Influence of membrane potential on the sodium-dependent uptake of gamma-aminobutyric acid by presynaptic nerve terminals: Experimental observations and theoretical considerations. J. Membrance Biol. 30:153–173.

    Google Scholar 

  7. Kontro, P., and Oja, S.S. 1978. Sodium dependence of taurine uptake in rat brain synaptosomes. Neuroscience 3:761–765.

    Google Scholar 

  8. Kanner, B.I. 1978. Active transport of γ-aminobutyric acid by membrane vesicles isolated from rat brain. Biochemistry, 17:1207–1211.

    Google Scholar 

  9. Kanner, B.I. 1980. Modulation of neurotransmiter transport by the activity of the action potential sodium ion channel in membrane vesicles from rat brain. Biochemistry, 19:692–697.

    Google Scholar 

  10. Kanner, B.I., and Kifer, L. 1981. Effluxe of γ-aminobutyric acid by membrane vesicles isolated from rat brain. Biochemistry, 20:3354–3358.

    Google Scholar 

  11. Ketternmann, H., and Schachner, M. 1985. Pharmacological properties of γ-aminobutyric acid, glutamate and aspartate induced depolarization in cultured astrocytes. J. Neurosci. 5:3295–3301.

    Google Scholar 

  12. Lowry, O.H., Rosebrough, N.J., Farr, A. L., and Randall, R.J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    Google Scholar 

  13. Halvarsson, G.B., Karlsson, I., and Sellström, Å. 1985. The use of3H-γ-aminobutyric acid for transport studies with isolated nerveterminals from rat brain. Life Sci. 37:209–216.

    Google Scholar 

  14. Zivin, J. A., and Waud, D. R. 1982. How to analyze binding enzyme and uptake data: The simplest case a single phase. Life Sci. 30:1407–1422.

    Google Scholar 

  15. Seeds, N.W., Gilman, A.G., Amano, T., and Nirenberg, M.W. 1970. Regulation of axon formation by clonal lines of a neuronal tumor. Proc. Natl. Acad. Sci. USA, 66:160–167.

    Google Scholar 

  16. Schubert, D., Humphreys, S., DeVitry, F., and Jacob, F. 1971. Induced differentiation of a neuroblastoma. Dev. Biol. 25:514–546.

    Google Scholar 

  17. Nelson, P., Ruffner, W., and Nirenberg, M. 1969. Neuronal tumor cells with exitable membranes grown in vitro. Proc. Natl. Acad. Sci. USA, 64:1004–1010.

    Google Scholar 

  18. Harris, A.J., and Dennis, M.J. 1970. Acetylcholine sensitivity and distribution on mouse neuroblastoma cells. Science, 167:1253–1255.

    Google Scholar 

  19. Spoerri, P.E., Glees, P., and Dresp, W. 1980. The time course of synapse formation of mouse neuroblastoma cells in monolayer cultures. Cell Tissue Res. 205:411–421.

    Google Scholar 

  20. Furmanski, P., Silverman, D.J., and Lubin, M. 1971. Experession of differentiated functions in mouse neuroblastoma mediated by di-butyrylcyclic adenosine monophosphate. Nature, 233:413–415.

    Google Scholar 

  21. Richelson, E. 1973. Regulation of tyrosine hydroxylase activity in mouse neuroblastoma clone NIE-115. J. Neurochem. 21:1139–1145.

    Google Scholar 

  22. Williams, L.R., Sandra, A., Sandquist, D., and Williams, T.H. 1981. Acetyltransferase activity and cytochmical localization in differentiating neuroblastoma. Cell Differ. 10:289–298.

    Google Scholar 

  23. Holopainen, I., Kontro, P., Frey, H.J., and Oja, S.S. 1983. Taurine, hypotaurine and GABA uptake by cultured neuroblastoma cells. J. Neurosci. Res. 10:83–92.

    Google Scholar 

  24. Holopainen, I., Kontro, P., and Oja, S.S. 1984. Taurine and hypotaurine transport in neuroblastoma cells: Effects of cations. Neurochem. Int. 6:217–222.

    Google Scholar 

  25. Sellström, Å., Venema, R., and Henn, F.A. 1976. Functional assessment of GABA uptake or exchange by synaptosomal fractions. Nature, 264:652–653.

    Google Scholar 

  26. Sihra, T.S., Scott, I.G., and Nicolls, D.G. 1984. Ionophore A 23187, Verapamil, and veratridine influence the release of γ-aminobutyric acid from synaptosomes by modulation of the plasma membrane potential rather than the cytosolic calcium. J. Neurochem. 43:1624–1630.

    Google Scholar 

  27. Holopainen, I., Kontro, P., and Oja, S.S. 1985. Release of preloaded taurine and hypotaurine from astrocytes in primary culture: Stimulation by calcium-free media. Neurochem. Res. 10:15–23.

    Google Scholar 

  28. Kontro, P. 1979. Components of taurine efflux in rat brain synaptosomes. Neuroscience, 4:1745–1749.

    Google Scholar 

  29. Korpi, E.R., and Oja, S.S. 1983. Characteristics of taurine release from cerebral cortex slices induced by sodium deficient media. Brain Res. 289:197–204.

    Google Scholar 

  30. Korpi, E.R., and Oja, S.S. 1984. Calcium chelators enhance the efflux of taurine from brain slices. Neuropharmacology, 23:377–380.

    Google Scholar 

  31. Prasad, K.N. 1975. Differentiation of neuroblastoma cells in culture. Biol. Rev. 50:129–165.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, University of Tampere, Box 607, SF-33101, Tampere 10, Finland

    Irma Holopainen

  2. Division of Experimental Medicine, National Defence Research Institute, S-901 82, Umeå, Sweden

    Eva Lidén, Åke Nilsson & Åke Sellström

Authors
  1. Irma Holopainen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Eva Lidén
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Åke Nilsson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Åke Sellström
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Holopainen, I., Lidén, E., Nilsson, Å. et al. Depolarization of the neuronal membrane caused by Co-transport of taurine and sodium. Neurochem Res 15, 89–94 (1990). https://doi.org/10.1007/BF00969189

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00969189

Key Words

Search

Navigation