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Lack of interaction between α2 and dopamine D2-receptors in mediating their inhibitory effects on [3H]dopamine release from rat nucleus accumbens slices

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Abstract

The α2-adrenoceptor agonist, UK14304, dose-dependently inhibited the electrically stimulated release of dopamine (DA) from rat nucleus accumbens slices. This effect was antagonized by idazoxan, confirming that it was an α2-adrenoceptor mediated effect. There was no evidence of endogenous activation of noradrenergic receptors suggesting that the α2-adrenoceptor agonist was not acting presynaptically to inhibit noradrenaline release. An in vitro superfusion technique was used to investigate wheher there was any interaction between α2-adrenoceptors and DA D2-receptors in mediating their inhibitory effects on [3H]DA release from rat nucleus accumbens slices. α2-Adrenoceptor and DA D2-receptors interact with similar second messenger systems and it was considered that they may compete for a common pool of G-proteins. The inhibitory effects of the α2-adrenoceptor agonist, UK14304, and the DA receptor agonists, quinpirole, apomorphine and pergolide were not independent. However, there was no evidence of any interaction between UK14304 and the DA D2-receptor antagonists, sulpiride or haloperidol, suggesting that the two receptors do not compete for a common pool of G-proteins in mediating their inhibitory effects on DA release.

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Authors and Affiliations

  1. MRC Research Unit for the Neurochemistry of Mental Diseases, Department of Chemical Pathology, University of Stellenbosch, Tygerberg Hospital, P O Box 19113, 7505, Tygerberg, (R.S.A.)

    V. A. Russell, M. C. L. Lamm & J. J. F. Taljaard

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  1. V. A. Russell
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  2. M. C. L. Lamm
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  3. J. J. F. Taljaard
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Russell, V.A., Lamm, M.C.L. & Taljaard, J.J.F. Lack of interaction between α2 and dopamine D2-receptors in mediating their inhibitory effects on [3H]dopamine release from rat nucleus accumbens slices. Neurochem Res 18, 285–289 (1993). https://doi.org/10.1007/BF00969084

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