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The contribution from the choroid plexus and the periventricular CNS to amino acids and proteins in the human CSF

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Abstract

During neurosurgery the freshly secreted extracellular fluid (ECF) from the choroid plexus was sampled with small pieces of application paper in three patients with intractable epilepsy. The samples were analyzed for free amino acids and for soluble proteins. The results were compared with corresponding data on extracellular fluid from the brain surface obtained with dialysis-perfusion as well as with the cerebrospinal fluid (CSF) acquired by lumbar punction. The dialysis data were calibrated against the paper results. The choroid plexus secretion had a high concentration of transthyretin as well as of an unidentified protein with an isoelectric point of 7.4. The cortical ECF exhibited high concentrations of tau-globulin and gamma-trace protein. Among the amino acids, glutamine had lower concentration in the choroid plexus secretion and higher concentrations in the ECF of the brain compared to the CSF. The amino acid derivative ethanolamine exhibited a similar pattern. This was interpreted to demonstrate that these compounds enter the CSF from the brain tissue. In contrast, alanine, serine, and taurine had a lower concentration in the CSF than in the plexus secretion which suggests that they are removed from the CSF by brain tissue.

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Authors and Affiliations

  1. Department of Neurobiology, University of Göteborg, Box 33031, S-400 33, Göteborg, Sweden

    Anders Hamberger & Britta Nyström

  2. Department of Neurosurgery, University of Göteborg, Box 33031, S-400 33, Göteborg, Sweden

    Herbert Silvenius

  3. Department of Neurology, University of Göteborg, Box 33031, S-400 33, Göteborg, Sweden

    Carsten Wikkelsø

Authors
  1. Anders Hamberger
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  2. Britta Nyström
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  3. Herbert Silvenius
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  4. Carsten Wikkelsø
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Hamberger, A., Nyström, B., Silvenius, H. et al. The contribution from the choroid plexus and the periventricular CNS to amino acids and proteins in the human CSF. Neurochem Res 15, 307–312 (1990). https://doi.org/10.1007/BF00968677

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  • DOI: https://doi.org/10.1007/BF00968677

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