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Metabolism of [1-13C]glucose in a synaptosomally enriched fraction of rat cerebrum studied by1H/13C magnetic resonance spectroscopy

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Abstract

This study explored the utility of1H and13C magnetic resonance spectroscopy to study a standard synaptosomally enriched fraction (P2 pellet) made from rat cerebrum. The preparations contained high concentrations of N-acetylaspartate and γ-aminobutyric acid and low concentrations of glutamine, indicating that they were in fact rich in neuronal cytosol. The metabolic competence of the preparation was assessed by quantitative measurements of its ability to convert [1-13C]glucose into lactate, glutamate, aspartate, and other metabolites under well oxygenated conditions in 30 minutes. The minimum mean glycolytic rate was 0.8 mM glucose/min and the flow through the tricarboxylic acid cycle was equivalent to 0.2 mM glucose/min.

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Abbreviations

ppm:

parts per million (chemical shift scale)

NMR:

nuclear magnetic resonance

GABA:

γ-aminobutyric acid

PBS:

phosphate-buffered normal saline solution

TSP:

3-trimethylsilylpropionate

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Authors and Affiliations

  1. Department of Neurology, Yale University, 333 Cedar St., 06510, New Haven, Connecticut, USA

    Ognen A. C. Petroff, Joel Black & James W. Prichard

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  1. Ognen A. C. Petroff
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  2. Alessandro P. Burlina
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  3. Joel Black
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  4. James W. Prichard
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Additional information

During the performance of these studies Dr. A.P. Burlina was on leave from Instituto di Clinica delle Malattie Nervose e Mentali, University of Padua, Padua, Italy.

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Petroff, O.A.C., Burlina, A.P., Black, J. et al. Metabolism of [1-13C]glucose in a synaptosomally enriched fraction of rat cerebrum studied by1H/13C magnetic resonance spectroscopy. Neurochem Res 16, 1245–1251 (1991). https://doi.org/10.1007/BF00966703

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