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Effect of hypoxia on mitochondrial protein composition of cerebral cortex during aging

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Abstract

The effect of hypoxia on the protein composition of mitochondria from cerebral cortex of rats at 4, 12, and 24 months of age was investigated. The proteins were separated by electrophoresis on SDS polyacrilamide gels and the percent content was evaluated by measuring the optical density of the stained gels. The results demonstrate that hypoxic treatment causes a decrease in the amount of some proteins as follows: the 90 and the 16 kDa Mw proteins at 4 months; the 82 and the 79 kDa Mw proteins at 24 months; the 52-49, 35 and 20 kDa at all ages investigated; the 44 kDa protein at 4 and 12 months and the 28 kDa protein at 4 and 24 months of age. Our results show that hypoxic conditions affect mitochondrial protein composition to a greater extent than aging alone.

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Authors and Affiliations

  1. Institute of Pharmacology, Faculty of Science, University of Pavia, Pavia, Italy

    Roberto F. Villa & Antonella Gorini

  2. Department of Biochemistry, University of Oulu, Oulu, Finland

    Leena Turpeenoja

  3. Institute of Biochemistry, Faculty of Medicine, University of Catania, 95125, Catania, Italy

    Grazia Magrì, Nicolò Ragusa & Anna Maria Giuffrida-Stella

Authors
  1. Roberto F. Villa
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  2. Leena Turpeenoja
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  3. Grazia Magrì
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  4. Antonella Gorini
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  5. Nicolò Ragusa
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  6. Anna Maria Giuffrida-Stella
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Special issue dedicated to Dr. Santiago Grisolia

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Villa, R.F., Turpeenoja, L., Magrì, G. et al. Effect of hypoxia on mitochondrial protein composition of cerebral cortex during aging. Neurochem Res 16, 821–826 (1991). https://doi.org/10.1007/BF00965692

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